关键信息
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基因名
KCNK3
- 应用
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别名
KCNK3；TASK；TASK1；Potassium channel subfamily K member 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O14649
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表达区间
1-394aa
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氨基酸序列
MKRQNVRTLA LIVCTFTYLL VGAAVFDALE SEPELIERQR LELRQQELRA RYNLSQGGYE ELERVVLRLK PHKAGVQWRF AGSFYFAITV ITTIGYGHAA PSTDGGKVFC MFYALLGIPL TLVMFQSLGE RINTLVRYLL HRAKKGLGMR RADVSMANMV LIGFFSCIST LCIGAAAFSH YEHWTFFQAY YYCFITLTTI GFGDYVALQK DQALQTQPQY VAFSFVYILT GLTVIGAFLN LVVLRFMTMN AEDEKRDAEH RALLTRNGQA GGGGGGGSAH TTDTASSTAA AGGGGFRNVY AEVLHFQSMC SCLWYKSREK LQYSIPMIIP RDLSTSDTCV EQSHSSPGGG GRYSDTPSRR CLCSGAPRSA ISSVSTGLHS LSTFRGLMKR RSSV
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分子量
51 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
KCNK3 (Potassium Channel, Subfamily K, Member 3) is a member of the two-pore domain potassium channel family, which plays a crucial role in regulating cellular excitability and ionic homeostasis. Mutations and dysregulation of KCNK3 have been implicated in various physiological and pathological conditions, including pulmonary arterial hypertension, where it contributes to the pulmonary vascular tone. The study of KCNK3 recombinant proteins has gained traction due to their potential therapeutic implications. Researchers aim to understand the channel's molecular mechanisms, regulatory pathways, and interaction with other cellular components. This knowledge is vital for the development of targeted therapies that could modulate channel activity for treating related diseases. Given that KCNK3 can be expressed in different cellular contexts and demonstrate distinct functional properties, the use of recombinant proteins allows for in-depth functional assays and characterization. By examining KCNK3's biophysical properties, pharmacological profiles, and functional roles in various cell types, scientists hope to uncover new insights that could lead to innovative treatment approaches for conditions influenced by potassium channel dysfunction. The ongoing research into KCNK3 recombinant proteins highlights the importance of ion channels in health and disease and the potential for channel-targeted therapies in clinical settings.
