| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA1161 |
| Uniprot No | Q6NSJ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-714aa |
| 活性数据 | MLQIPQEKSQAYPRRRRPGCYAYRQNPEAIAAAAMYTFLPDNFSPAKPKPSKELKPLLGSAVLGLLLVLAAVVAWCYYSVSLRKAERLRAELLDLKAGGFSIRNQKGEQVFRLAFRSGALDLDSCSRDGALLGCSLTADGLPLHFFIQTVRPKDTVMCYRVRWEEAAPGRAVEHAMFLGDAAAHWYGGAEMRTQHWPIRLDGQQEPQPFVTSDVYSSDAAFGGILERYWLSSRAAAIKVNDSVPFHLGWNSTERSLRLQARYHDTPYKPPAGRAAAPELSYRVCVGSDVTSIHKYMVRRYFNKPSRVPAPEAFRDPIWSTWALYGRAVDQDKVLRFAQQIRLHHFNSSHLEIDDMYTPAYGDFDFDEVKFPNASDMFRRLRDAGFRVTLWVHPFVNYNSSRFGEGVERELFVREPTGRLPALVRWWNGIGAVLDFTHPKARDWFQGHLRRLRSRYSVASFKFDAGEVSYLPRDFSTYRPLPDPSVWSRRYTEMALPFFSLAEVRVGYQSQNISCFFRLVDRDSVWGYDLGLRSLIPAVLTVSMLGYPFILPDMVGGNAVPQRTAGGDVPERELYIRWLEVAAFMPAMQFSIPPWRYDAEVVAIAQKFAALRASLVAPLLLELAGEVTDTGDPIVRPLWWIAPGDETAHRIDSQFLIGDTLLVAPVLEPGKQERDVYLPAGKWRSYKGELFDKTPVLLTDYPVDLDEIAYFTWAS |
| 分子量 | 107.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA1161蛋白的3篇参考文献示例(部分为虚构，仅供参考)：
1. **文献名称**：*Expression and Functional Characterization of Recombinant Human KIAA1161 Protein in Mammalian Cells*
**作者**：Chen L, et al.
**摘要**：本研究成功克隆并在HEK293细胞中表达了重组人KIAA1161蛋白，证实其具有跨膜结构域，并通过免疫印迹和质谱验证蛋白纯度。功能实验表明，KIAA1161可能参与调控细胞黏附过程。
2. **文献名称**：*KIAA1161 Modulates Neuronal Axon Guidance via Interaction with Collagen VI*
**作者**：Wang Y, et al.
**摘要**：通过体外重组KIAA1161蛋白与胶原蛋白VI的互作实验，发现两者结合后可激活下游MAPK信号通路，促进神经元轴突导向，提示其在神经系统发育中的作用。
3. **文献名称**：*Proteomic Analysis of KIAA1161-Associated Proteins in Cancer Cell Invasion*
**作者**：Sato K, et al.
**摘要**：利用重组KIAA1161蛋白进行免疫共沉淀结合质谱分析，鉴定出多个肿瘤转移相关蛋白(如Integrin β1)，并发现KIAA1161高表达可抑制肺癌细胞侵袭能力。
**备注**：以上文献为模拟示例，实际研究中KIAA1161相关报道较少，建议通过PubMed或Web of Science以“KIAA1161”、“VGLL4”(可能的别名)或“recombinant protein”为关键词检索最新文献。
Recombinant human KIAA1161 protein is a genetically engineered variant of the KIAA1161 protein, encoded by the KIAA1161 gene located on chromosome 2q37.3. Initially identified through large-scale sequencing projects, KIAA1161 is a poorly characterized protein with limited functional data. Bioinformatic analyses suggest it contains conserved coiled-coil domains and transmembrane regions, hinting at roles in intracellular trafficking, membrane dynamics, or organelle organization. Studies link it to cellular processes like autophagy and lysosomal function, with potential implications in neurological disorders and cancer. Its low basal expression in most tissues and upregulated presence in specific tumors have sparked interest in its oncogenic or tumor-suppressive properties.
The recombinant form is typically produced in mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications. Purification via affinity tags enables its use in functional studies, antibody development, and structural analysis. Current research focuses on clarifying its interactome, subcellular localization, and mechanistic contributions to diseases. As a research tool, recombinant KIAA1161 aids in exploring its physiological role and therapeutic potential, particularly in contexts involving vesicle transport or autophagy dysregulation. Further investigations are needed to fully decipher its biological significance and clinical relevance.
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