| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0265 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-442aa |
| 活性数据 | MSAAQGWDRNRRRGGGAAGAGGGGSGAGGGSGGSGGRGTGQLNRFVQLSGRPHLPGKKKIRWDPVRRRFIQSCPIIRIPNRFLRGHRPPPARSGHRCVADNTNLYVFGGYNPDYDESGGPDNEDYPLFRELWRYHFATGVWHQMGTDGYMPRELASMSLVLHGNNLLVFGGTGIPFGESNGNGVHVCNVKYKRWALLSCRGKKPSRIYGQAMAIINGSLYVFGGTTGYIYSTDLHKLDLNTREWTQLKPNNLSCDLPEERYRHEIAHDGQRIYVLGGGTSWTAYSLNKIHAYNLGTNAWEEIATKPHEKIGFPAARRCHSCVQIKNDVFICGGYNGEVILGDIWKLNLQTFQWVKLPATMPEPVYFHCAAVTPAGCMYIHGGVVNIHENKRTGSLFKIWLVVPSLLELAWEKLLAAFPNLANLSRTQLLHLGLTQGLIERLK |
| 分子量 | 75.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0265蛋白的模拟参考文献示例(请注意，这些文献为虚构示例，实际研究中可能需要通过学术数据库进一步检索)：
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1. **文献名称**：*Molecular Cloning and Functional Analysis of Recombinant Human KIAA0265 Protein*
**作者**：Zhang L, Wang Y, Chen H
**摘要**：本研究成功在大肠杆菌系统中表达并纯化了重组人KIAA0265蛋白，通过质谱和Western blot验证其正确折叠。功能实验表明，KIAA0265在体外与微管蛋白结合，暗示其在细胞骨架调控中的作用。
2. **文献名称**：*KIAA0265 Interacts with Apoptosis Signal-Regulating Kinase 1 (ASK1) in a Stress Response Pathway*
**作者**：Tanaka R, Ito S
**摘要**：利用哺乳动物细胞表达系统制备重组KIAA0265蛋白，并证实其通过磷酸化修饰与ASK1结合。实验表明，KIAA0265可能作为适配蛋白调控JNK/p38信号通路。
3. **文献名称**：*Crystal Structure of the Recombinant KIAA0265 C-terminal Domain Reveals a Novel Ubiquitin-Binding Motif*
**作者**：Kim J, Park SM
**摘要**：通过昆虫细胞表达系统获得重组KIAA0265蛋白的C端结构域，解析其晶体结构(分辨率为2.1 Å)，并发现其含有一个新型泛素结合基序，提示其在蛋白质降解中的潜在功能。
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**建议**：实际文献需通过PubMed、Google Scholar或UniProt(条目号：Q9H6T3/KIAA0265)检索。例如，UniProt中列出KIAA0265(现称**GPAT4**)可能参与甘油磷脂代谢，相关研究文献可通过基因别名“GPAT4”进一步查找。
Recombinant human KIAA0265 protein is a genetically engineered version of the protein encoded by the KIAA0265 gene, which was initially identified through large-scale sequencing efforts of the human genome. This gene, also known as RAB6B, belongs to the Ras-associated binding (RAB) GTPase family, a group of small GTPases involved in regulating intracellular vesicle trafficking and membrane transport. The KIAA0265 protein is predicted to play roles in specific secretory pathways or Golgi-ER communication, though its precise biological functions remain under investigation. Its association with RAB6 isoforms suggests potential involvement in neuronal or endocrine processes, given the established roles of RAB6 proteins in neurotransmitter release and hormone secretion. Recombinant expression systems, such as Escherichia coli or mammalian cell lines, enable the production of purified KIAA0265 protein for functional studies, antibody development, and interaction mapping. Researchers utilize this tool to explore its subcellular localization, GTPase activity, and possible links to diseases like cancer or neurodegenerative disorders. Structural and biochemical analyses of the recombinant protein contribute to elucidating its mechanism of action and regulatory networks within the RAB family.
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