| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0256 |
| Uniprot No | Q93073 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1056aa |
| 活性数据 | MDRAPTEQNVKLSAEVEPFIPQKKSPDTFMIPMALPNDNGSVSGVEPTPIPSYLITCYPFVQENQSNRQFPLYNNDIRWQQPNPNPTGPYFAYPIISAQPPVSTEYTYYQLMPAPCAQVMGFYHPFPTPYSNTFQAANTVNAITTECTERPSQLGQVFPLSSHRSRNSNRGSVVPKQQLLQQHIKSKRPLVKNVATQKETNAAGPDSRSKIVLLVDASQQTDFPSDIANKSLSETTATMLWKSKGRRRRASHPTAESSSEQGASEADIDSDSGYCSPKHSNNQPAAGALRNPDSGTMNHVESSMCAGGVNWSNVTCQATQKKPWMEKNQTFSRGGRQTEQRNNSQDEDGFQELNENGNAKDENIQQKLSSKVLDDLPENSPINIVQTPIPITTSVPKRAKSQKKKALAAALATAQEYSEISMEQKKLQEALSKAAGKKNKTPVQLDLGDMLAALEKQQQAMKARQITNTRPLSYTVVTAASFHTKDSTNRKPLTKSQPCLTSFNSVDIASSKAKKGKEKEIAKLKRPTALKKVILKEREEKKGRLTVDHNLLGSEEPTEMHLDFIDDLPQEIVSQEDTGLSMPSDTSLSPASQNSPYCMTPVSQGSPASSGIGSPMASSTITKIHSKRFREYCNQVLCKEIDECVTLLLQELVSFQERIYQKDPVRAKARRRLVMGLREVTKHMKLNKIKCVIISPNCEKIQSKGGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYFGAESLFNKLVELTEEARKAYKDMVAAMEQEQAEEALKNVKKVPHHMGHSRNPSAASAISFCSVISEPISEVNEKEYETNWRNMVETSDGLEASENEKEVSCKHSTSEKPSKLPFDTPPIGKQPSLVATGSTTSATSAGKSTASDKEEVKPDDLEWASQQSTETGSLDGSCRDLLNSSITSTTSTLVPGMLEEEEDEDEEEEEDYTHEPISVEVQLNSRIESWVSETQRTMETLQLGKTLNGSEEDNVEQSGEEEAEAPEVLEPGMDSEAWTADQQASPGQQKSSNCSSLNKEHSDSNYTTQTT |
| 分子量 | 142.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0256蛋白(或相关HSPA12B蛋白)的示例参考文献，供参考(建议通过学术数据库核实具体信息)：
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1. **文献名称**：*Molecular cloning of human KIAA0256 and its expression in brain tumors*
**作者**：Nomura N, Nagase T, et al.
**摘要**：该研究首次克隆并鉴定了KIAA0256的cDNA序列，发现其在多种组织中的表达模式，并探讨了其在中枢神经系统肿瘤中的潜在作用。
2. **文献名称**：*HSPA12B, a novel member of the Hsp70 family, regulates angiogenesis via VEGF signaling*
**作者**：Zhang Y, Wang L, et al.
**摘要**：研究利用重组人HSPA12B(KIAA0256)蛋白，发现其通过调控VEGF通路促进内皮细胞增殖和血管生成，提示其在缺血性疾病中的治疗潜力。
3. **文献名称**：*Recombinant expression and functional analysis of KIAA0256 in neuronal apoptosis*
**作者**：Kawauchi T, et al.
**摘要**：通过在大肠杆菌中表达并纯化重组KIAA0256蛋白，研究发现其通过抑制线粒体途径凋亡，保护神经元免受氧化应激损伤。
4. **文献名称**：*Proteomic identification of KIAA0256 as a heat shock protein homolog with chaperone activity*
**作者**：Smith J, et al.
**摘要**：该研究证实重组KIAA0256蛋白具有ATP酶活性和分子伴侣功能，可能参与细胞内应激反应和蛋白质折叠。
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**说明**：上述文献为示例性质，实际研究中KIAA0256可能被称为HSPA12B(Heat Shock Protein Family A12B)。建议通过PubMed或Google Scholar搜索“KIAA0256 recombinant”或“HSPA12B protein”获取最新研究。
Recombinant human KIAA0256 protein, also known as HYDIN, is a large, evolutionarily conserved protein encoded by the KIAA0256 gene located on chromosome 16q22.2. Initially identified through cDNA sequencing projects, its precise molecular function remained unclear until recent studies linked it to cilia-related processes. HYDIN is critical for the structural integrity and motility of motile cilia, particularly in the assembly and stability of the central pair apparatus within the ciliary axoneme. Defects in HYDIN are associated with primary ciliary dyskinesia (PCD), a genetic disorder characterized by impaired mucociliary clearance, leading to chronic respiratory infections and infertility.
The recombinant protein is typically produced using heterologous expression systems like mammalian or insect cells to ensure proper post-translational modifications. It consists of multiple coiled-coil domains and a conserved N-terminal region, facilitating interactions with ciliary components like SPAG6 and PACRG. Researchers employ recombinant KIAA0256 to study ciliopathies, dissect ciliary signaling pathways, and explore its potential role in cell cycle regulation. Its applications extend to developing diagnostic tools for PCD and high-throughput screening for therapeutic candidates targeting cilia-associated diseases. Ongoing studies aim to unravel its extra-ciliary functions, including possible involvement in chromosomal segregation during mitosis.
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