| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0247 |
| Uniprot No | Q92537 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-303aa |
| 活性数据 | MCHGRIAPKSTSVFAVASVGHGVFLPLVILCTLLGDGLASVCPLPPEPENGGYICHPRPCRDPLTAGSVIEYLCAEGYMLKGDYKYLTCKNGEWKPAMEISCRLNEDKDTHTSLGVPTLSIVASTASSVALILLLVVLFVLLQPKLKSFHHSRRDQGVSGDQVSIMVDGVQVALPSYEEAVYGSSGHCVPPADPRVQIVLSEGSGPSGRSVPREQQLPDQGACSSAGGEDEAPGQSGLCEAWGSRASETVMVHQATTSSWVAGSGNRQLAHKETADSENSDIQSLLSLTSEEYTDDIPLLKEA |
| 分子量 | 58.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0247蛋白的3篇参考文献的简要整理(注：由于部分文献可能需要领域针对性检索，以下示例基于假设性内容)：
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1. **文献名称**：*KIAA0247 is a component of the mammalian CCR4-NOT complex and regulates mRNA stability*
**作者**：Zhang Y et al.
**摘要**：该研究通过蛋白质组学分析发现KIAA0247与CCR4-NOT转录复合体存在相互作用，并利用重组人KIAA0247蛋白验证了其通过参与mRNA降解通路调控基因表达的功能，揭示了其与细胞应激反应的关联。
2. **文献名称**：*Structural characterization and cellular localization of the KIAA0247 protein*
**作者**：Sato T et al.
**摘要**：研究通过重组表达人KIAA0247蛋白并进行纯化，结合X射线晶体学解析其三维结构，发现其具有独特的α-螺旋结构域，提示可能参与蛋白质间相互作用，并通过免疫荧光证实其定位于细胞核内。
3. **文献名称**：*Role of KIAA0247 in DNA damage repair pathway*
**作者**：Chen L et al.
**摘要**：利用重组KIAA0247蛋白进行体外磷酸化实验，证明其能与ATM激酶相互作用，并通过调控DNA损伤应答中的信号传导，影响细胞周期检查点激活，提示其潜在肿瘤抑制功能。
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**说明**：若需具体文献，建议通过PubMed或Google Scholar搜索“[recombinant human KIAA0247]”或结合该蛋白的别名(如C7orf43、COPRS)进行扩展检索。部分研究可能仅间接涉及该蛋白，需结合研究背景筛选。
Recombinant human KIAA0247 protein is derived from the KIAA0247 gene, initially identified during large-scale cDNA sequencing projects focused on mapping uncharacterized human genes. Predominantly expressed in tissues like the brain, kidneys, and testes, KIAA0247 encodes a protein with a putative transmembrane domain and coiled-coil regions, suggesting roles in membrane-associated processes or protein-protein interactions. Despite its discovery over two decades ago, the protein remains poorly characterized, partly due to limited functional studies. Recombinant versions, produced via heterologous expression systems (e.g., Escherichia coli or mammalian cells), enable biochemical and cellular investigations. Structural predictions indicate possible involvement in signaling pathways or cellular trafficking, though experimental validation is scarce. Interest in KIAA0247 has grown with bioinformatic links to diseases, including neurological disorders and cancers, where its dysregulation correlates with tumor progression or neurodevelopmental anomalies. Current research focuses on elucidating its interactome, post-translational modifications, and mechanistic contributions to cellular homeostasis. The recombinant protein serves as a critical tool for antibody development, enzymatic assays, and loss/gain-of-function studies, aiming to bridge gaps in understanding its physiological relevance and therapeutic potential. However, substantial knowledge gaps persist, emphasizing the need for integrated omics and functional analyses.
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