| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KDELR3 |
| Uniprot No | O43731 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-214aa |
| 活性数据 | MNVFRILGDLSHLLAMILLLGKIWRSKCCKGISGKSQILFALVFTTRYLDLFTNFISIYNTVMKVVFLLCAYVTVYMIYGKFRKTFDSENDTFRLEFLLVPVIGLSFLENYSFTLLEILWTFSIYLESVAILPQLFMISKTGEAETITTHYLFFLGLYRALYLANWIRRYQTENFYDQIAVVSGVVQTIFYCDFFYLYVTKVLKGKKLSLPMPI |
| 分子量 | 49.28 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人KDELR3蛋白相关的参考文献示例(注：文献信息为模拟示例，需核实真实文献后再引用)：
1. **标题**：*Structural insights into KDEL receptor 3-mediated retrograde transport*
**作者**：Smith A, et al.
**摘要**：通过X射线晶体学解析重组人KDELR3蛋白的构象，揭示其与内质网驻留蛋白KDEL基序结合的特异性机制，阐述其在逆向转运中的作用。
2. **标题**：*KDELR3 modulates ER stress response via interaction with HSPA5 in cancer cells*
**作者**：Li X, et al.
**摘要**：研究重组人KDELR3蛋白与分子伴侣HSPA5的互作，发现其在调控内质网应激和肿瘤细胞存活中的关键功能。
3. **标题**：*Functional characterization of recombinant KDEL receptors in COPI vesicle trafficking*
**作者**：Yamamoto K, et al.
**摘要**：利用重组KDELR3蛋白进行体外实验，验证其招募COPI囊泡并介导内质网回收通路的分子机制。
4. **标题**：*Expression and purification of recombinant human KDELR3 for drug screening assays*
**作者**：Chen L, et al.
**摘要**：建立重组KDELR3蛋白的原核表达及纯化方法，应用于高通量筛选靶向该受体的潜在药物分子。
建议通过 **PubMed** 或 **Web of Science** 搜索真实文献，筛选以"KDELR3"、"recombinant protein"为关键词的研究论文及结构/功能分析类文章。
KDELR3. a member of the KDEL receptor family, is a Golgi-localized transmembrane protein responsible for retrieving endoplasmic reticulum (ER)-resident proteins bearing a C-terminal KDEL-like motif (e.g., BiP, GRP94) from the secretory pathway. It ensures ER homeostasis by orchestrating the retrograde transport of escaped chaperones via COPI-coated vesicles. Structurally, KDELR3 contains seven transmembrane domains and interacts with G proteins, modulating downstream signaling pathways like cAMP and MAPK.
Recombinant human KDELR3 protein is engineered using mammalian or bacterial expression systems, enabling in vitro studies of its trafficking mechanisms and ligand-binding specificity. Research highlights its role in cellular stress responses, protein quality control, and diseases like cancer and neurodegeneration. Overexpression of KDELR3 has been linked to tumor progression, likely by enhancing cell survival under ER stress. Additionally, its involvement in viral pathogenesis (e.g., coronaviruses hijacking ER retention signals) underscores its biomedical relevance.
Current studies utilize recombinant KDELR3 to explore therapeutic strategies targeting ER stress pathways or intracellular trafficking. Its functional versatility makes it a pivotal molecule in understanding secretory pathway regulation and developing interventions for protein-misfolding disorders.
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