| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KHDRBS3 |
| Uniprot No | O75525 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-346aa |
| 活性数据 | MEEKYLPELMAEKDSLDPSFTHALRLVNQEIEKFQKGEGKDEEKYIDVVINKNMKLGQKVLIPVKQFPKFNFVGKLLGPRGNSLKRLQEETLTKMSILGKGSMRDKAKEEELRKSGEAKYFHLNDDLHVLIEVFAPPAEAYARMGHALEEIKKFLIPDYNDEIRQAQLQELTYLNGGSENADVPVVRGKPTLRTRGVPAPAITRGRGGVTARPVGVVVPRGTPTPRGVLSTRGPVSRGRGLLTPRARGVPPTGYRPPPPPPTQETYGEYDYDDGYGTAYDEQSYDSYDNSYSTPAQSGADYYDYGHGLSEETYDSYGQEEWTNSRHKAPSARTAKGVYRDQPYGRY |
| 分子量 | 65.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人KHDRBS3蛋白的参考文献示例(内容基于虚构研究归纳，供参考格式)：
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1. **文献名称**: *KHDRBS3 promotes tumorigenesis in colorectal cancer via regulating mRNA stability*
**作者**: Zhang L, et al.
**摘要**: 该研究通过重组表达人KHDRBS3蛋白，发现其通过结合靶基因(如Cyclin D1)的mRNA 3'UTR增强稳定性，促进结直肠癌细胞增殖，提示其在癌症中的致癌作用。
2. **文献名称**: *Structural insights into RNA recognition by KHDRBS3 protein*
**作者**: Müller S, et al.
**摘要**: 利用重组KHDRBS3蛋白的晶体结构分析，揭示了其KH结构域与特定RNA序列的结合机制，为RNA剪接调控研究提供结构基础。
3. **文献名称**: *KHDRBS3 deficiency disrupts synaptic plasticity in a mouse model of neurodevelopmental disorders*
**作者**: Wang Y, et al.
**摘要**: 通过重组蛋白拯救实验，证实KHDRBS3缺失会导致海马神经元突触蛋白合成异常，可能与自闭症相关神经发育障碍有关。
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**备注**：上述文献为示例，实际文献需通过PubMed或Google Scholar检索关键词如“KHDRBS3 recombinant protein”获取。如需真实文献，建议查阅相关学术数据库。
KHDRBS3 (KH RNA Binding Domain Containing, Signal Transduction Associated 3), also known as Src-associated in mitosis 68 kDa protein 3 (Sam68-like protein 1/SLM1), is a member of the STAR (Signal Transduction and Activation of RNA) family. This protein is evolutionarily conserved and primarily functions as an RNA-binding protein involved in post-transcriptional gene regulation. It contains a KH (heterogeneous nuclear ribonucleoprotein K homology) domain, enabling interactions with RNA motifs, particularly AU-rich elements in mRNA targets. KHDRBS3 participates in alternative splicing, mRNA stability, and translation by recruiting regulatory complexes through signal transduction pathways, such as MAPK/ERK or SRC kinase cascades.
Biologically, KHDRBS3 is implicated in cellular processes like cell cycle progression, apoptosis, and differentiation. Dysregulation of KHDRBS3 has been associated with cancer progression, neurological disorders, and infertility. For instance, aberrant expression correlates with tumorigenesis in prostate, breast, and colorectal cancers, likely by modulating oncogenic mRNA networks. Recombinant human KHDRBS3 protein is engineered for functional studies, enabling exploration of RNA-protein interactions, kinase-mediated phosphorylation effects, and therapeutic target validation. Produced via bacterial or mammalian expression systems, it retains RNA-binding activity and structural integrity for in vitro assays. Its recombinant form aids in elucidating molecular mechanisms underlying RNA metabolism and disease pathogenesis, offering potential for diagnostic or therapeutic applications.
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