| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KHSRP |
| Uniprot No | Q92945 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 151-239aa |
| 活性数据 | VPDGMVGLIIGRGGEQINKIQQDSGCKVQISPDSGGLPERSVSLTGAPESVQKAKMMLDDIVSRGRGGPPGQFHDNANGGQNGTVQEIM |
| 分子量 | 35.53 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**：KHSRP通过调控炎症因子mRNA稳定性参与先天免疫反应
**作者**：G. Stoecklin 等
**摘要**：本研究阐明了重组人KHSRP蛋白通过结合促炎细胞因子(如TNF-α、IL-8)mRNA的AU富集元件，促进其降解，从而负向调控炎症反应。实验利用重组KHSRP证实其对RNA分解复合物的招募作用。
2. **文献名称**：KHSRP与LIN28协同调控let-7 microRNA的生物发生
**作者**：R. I. Gregory 等
**摘要**：研究证明重组人KHSRP与LIN28蛋白在体外协同结合pre-let-7 RNA，抑制其成熟，影响干细胞多能性维持。重组蛋白实验揭示KHSRP在microRNA加工中的直接作用。
3. **文献名称**：KHSRP在胶质母细胞瘤中通过RNA代谢驱动侵袭性表型
**作者**：J. D. Ule 等
**摘要**：通过重组人KHSRP蛋白功能实验，发现其通过稳定促癌基因(如c-MYC)mRNA，增强肿瘤细胞迁移及侵袭能力，提示KHSRP作为潜在治疗靶点。
4. **文献名称**：KHSRP在神经元分化中的动态RNA结合调控网络
**作者**：S. A. Tenenbaum 等
**摘要**：研究利用重组KHSRP进行体外RNA结合分析，揭示其通过选择性稳定神经发育相关转录本(如NeuroD1)，调节神经元分化时序。
KHSRP (KH-type splicing regulatory protein), also known as KSRP, is a multifunctional RNA-binding protein involved in post-transcriptional gene regulation. It belongs to the evolutionarily conserved family of KH domain proteins, which are critical for RNA recognition and interaction. Structurally, KHSRP contains four tandem KH domains that facilitate binding to AU-rich elements (AREs) in the 3'-untranslated regions (3'UTRs) of numerous target mRNAs. This interaction plays a central role in regulating mRNA stability, degradation, and translation, particularly for transcripts encoding proteins involved in inflammation, cell proliferation, and stress responses.
Beyond mRNA metabolism, KHSRP participates in alternative pre-mRNA splicing and microRNA biogenesis by associating with the Drosha-DGCR8 complex. Its functional versatility extends to viral infection responses, where it may either promote or inhibit viral replication depending on the pathogen. Recombinant human KHSRP protein is typically produced using bacterial or mammalian expression systems, enabling studies on its RNA-binding properties, structural domains, and regulatory mechanisms. Researchers utilize this protein to dissect its roles in cellular processes, disease models (e.g., cancer, neurodegenerative disorders), and potential therapeutic targeting. Dysregulation of KHSRP has been linked to chronic inflammation, tumor progression, and neurological abnormalities, highlighting its biological and clinical relevance.
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