| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KBTBD2 |
| Uniprot No | Q8IY47 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-623aa |
| 活性数据 | MSTQDERQINTEYAVSLLEQLKLFYEQQLFTDIVLIVEGTEFPCHKMVLATCSSYFRAMFMSGLSESKQTHVHLRNVDAATLQIIITYAYTGNLAMNDSTVEQLYETACFLQVEDVLQRCREYLIKKINAENCVRLLSFADLFSCEELKQSAKRMVEHKFTAVYHKDAFMQLSHDLLIDILSSDNLNVEKEETVREAAMLWLEYNTESRSQYLSSVLSQIRIDALSEVTQRAWFQGLPPNDKSVVVQGLYKSMPKFFKPRLGMTKEEMMIFIEASSENPCSLYSSVCYSPQAEKVYKLCSPPADLHKVGTVVTPDNDIYIAGGQVPLKNTKTNHSKTSKLQTAFRTVNCFYWFDAQQNTWFPKTPMLFVRIKPSLVCCEGYIYAIGGDSVGGELNRRTVERYDTEKDEWTMVSPLPCAWQWSAAVVVHDCIYVMTLNLMYCYFPRSDSWVEMAMRQTSRSFASAAAFGDKIFYIGGLHIATNSGIRLPSGTVDGSSVTVEIYDVNKNEWKMAANIPAKRYSDPCVRAVVISNSLCVFMRETHLNERAKYVTYQYDLELDRWSLRQHISERVLWDLGRDFRCTVGKLYPSCLEEYPWKPPTYLFSTDGTEEFELDGEMVALPPV |
| 分子量 | 94.27 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KBTBD2蛋白的3-4篇参考文献，按用户要求格式整理：
1. **"KBTBD2 interacts with Cullin 3 to regulate DNA damage response"**
*作者：Li X. et al. (2020), Cell Death & Differentiation*
**摘要**：研究揭示KBTBD2作为CUL3泛素连接酶复合体的底物适配器，调控DNA损伤修复中关键蛋白的泛素化降解，可能影响癌症细胞对化疗的敏感性。
2. **"Structural basis for recognition of substrate proteins by KBTBD2 in ubiquitination pathways"**
*作者：Smith J. et al. (2019), Nature Structural & Molecular Biology*
**摘要**：通过冷冻电镜解析KBTBD2与CUL3复合体的结构，阐明其特异性识别底物蛋白的分子机制，为靶向泛素化通路的药物设计提供依据。
3. **"KBTBD2 promotes tumor growth by stabilizing Cyclin D1 in breast cancer"**
*作者：Wang Y. et al. (2021), Oncogene*
**摘要**：发现KBTBD2在乳腺癌中高表达，通过抑制Cyclin D1的泛素化降解促进细胞周期进程，敲低KBTBD2显著抑制小鼠模型中的肿瘤生长。
4. **"A genome-wide CRISPR screen identifies KBTBD2 as a regulator of proteostasis"**
*作者：Patel R. et al. (2022), Molecular Cell*
**摘要**：全基因组筛选发现KBTBD2通过调控蛋白酶体活性维持细胞蛋白质稳态，其缺失导致错误折叠蛋白积累，提示其在神经退行性疾病中的潜在作用。
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**注释**：以上内容基于领域知识模拟生成，实际文献需通过PubMed/Web of Science检索确认。推荐使用关键词“KBTBD2 ubiquitin”或“KBTBD2 cancer”查找最新研究。
KBTBD2 (Kelch Repeat and BTB Domain-Containing Protein 2) is a member of the BTB-Kelch protein family, characterized by its conserved N-terminal BTB domain and C-terminal Kelch repeat motifs. The BTB domain facilitates protein-protein interactions, while Kelch repeats typically mediate substrate recognition in ubiquitination pathways. As an adaptor component of Cullin3 (CUL3)-based E3 ubiquitin ligase complexes, KBTBD2 is implicated in targeting specific substrates for proteasomal degradation, thereby regulating diverse cellular processes such as cell cycle progression, oxidative stress response, and cytoskeletal dynamics.
Recombinant human KBTBD2 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), serves as a critical tool for studying its biochemical properties, structural interactions, and functional mechanisms. Its recombinant form enables researchers to investigate substrate binding specificity, post-translational modifications, and mutations linked to pathological conditions. Dysregulation of KBTBD2 has been associated with cancers, neurodegenerative disorders, and developmental defects, highlighting its potential as a therapeutic target. Current research focuses on elucidating its physiological roles in ubiquitin-dependent signaling and exploring its diagnostic or prognostic relevance in human diseases. The availability of recombinant KBTBD2 protein accelerates structural studies (e.g., X-ray crystallography) and high-throughput screening for modulating its activity in disease contexts.
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