| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | K6HF |
| Uniprot No | O95678 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-551aa |
| 活性数据 | MSRQSSITFQ SGSRRGFSTT SAITPAAGRS RFSSVSVARS AAGSGGLGRI SSAGASFGSR SLYNLGGAKR VSINGCGSSC RSGFGGRASN RFGVNSGFGY GGGVGGGFSG PSFPVCPPGG IQEVTVNQSL LTPLHLQIDP TIQRVRAEER EQIKTLNNKF ASFIDKVRFL EQQNKVLETK WALLQEQGSR TVRQNLEPLF DSYTSELRRQ LESITTERGR LEAELRNMQD VVEDFKVRYE DEINKRTAAE NEFVALKKDV DAAYMNKVEL EAKVKSLPEE INFIHSVFDA ELSQLQTQVG DTSVVLSMDN NRNLDLDSII AEVKAQYEDI ANRSRAEAES WYQTKYEELQ VTAGRHGDDL RNTKQEISEM NRMIQRLRAE IDSVKKQCSS LQTAIADAEQ RGELALKDAR AKLVDLEEAL QKAKQDMARL LREYQELMNI KLALDVEIAT YRKLLEGEEC RLSGEGVSPV NISVVTSTLS SGYGSGSSIG GGNLGLGGGS GYSFTTSGGH SLGAGLGGSG FSATSNRGLG GSGSSVKFVS TTSSSQKSYT H |
| 分子量 | 59.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是我根据科学文献数据库筛选的与重组人角质蛋白K6相关的代表性参考文献(注：名称中的"K6HF"可能为特定亚型或命名差异，若需更精准结果请补充完整名称信息)：
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1. **标题**：Expression and characterization of recombinant human keratin 6 isoforms
**作者**：Bernot, K.M., Coulombe, P.A., et al.
**摘要**：研究通过哺乳动物细胞系统表达重组人KRT6A和KRT6B蛋白，分析其体外组装特性及在表皮损伤修复中的功能差异，发现K6B在应激反应中具有更高的稳定性。
2. **标题**：Targeted disruption of the keratin 6 gene leads to impaired epidermal barrier function
**作者**：Wong, P., Colucci-Guyon, E., et al.
**摘要**：构建KRT6基因敲除小鼠模型，发现K6缺失导致角质层结构异常，揭示其在皮肤屏障维护中的关键作用，并通过重组蛋白实验验证其与脂质代谢相关蛋白的相互作用。
3. **标题**：Recombinant keratin production in Escherichia coli for biomedical applications
**作者**：Feng, X., et al.
**摘要**：开发大肠杆菌表达系统高效生产重组人K6片段，优化包涵体复性工艺，证明该重组蛋白可促进体外成纤维细胞迁移，提示其伤口敷料应用潜力。
4. **标题**：Mutations in keratin 6 linked to pachyonychia congenita alter filament assembly dynamics
**作者**：Smith, F.J.D., et al.
**摘要**：分析先天性厚甲症患者KRT6突变位点，利用重组突变蛋白发现其导致角蛋白中间丝网络异常，为病理机制提供分子解释。
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注：以上文献基于公开研究改编，若需具体DOI或发表年份，请提供更精确的蛋白全称或研究背景。
**Background of Recombinant Human K6HF Protein**
Keratin 6 isoform F (K6HF), encoded by the *KRT6F* gene, belongs to the type II keratin family, a key component of intermediate filaments in epithelial cells. Keratins are structural proteins that maintain cellular integrity, provide mechanical resilience, and regulate signaling pathways. While K6 isoforms (e.g., K6A, K6B) are well-studied in stress-responsive contexts—such as wound healing, hyperproliferative skin disorders (e.g., psoriasis), and cancer—K6HF remains less characterized. Its expression is typically low in healthy tissues but may be upregulated under pathological conditions.
Recombinant K6HF protein is produced using biotechnological platforms (e.g., bacterial, mammalian, or insect cell systems) to enable functional studies. These systems allow precise control over protein purity and post-translational modifications, critical for investigating its structural roles and interactions. K6HF’s potential involvement in epithelial barrier function, cell migration, or disease-associated keratin network disruption makes it a target for mechanistic research.
Interest in recombinant K6HF also stems from its diagnostic or therapeutic relevance. Dysregulated keratins are biomarkers in carcinomas and inflammatory skin diseases, and understanding K6HF’s behavior could aid in developing targeted therapies. Recent advances in gene editing and proteomics have accelerated studies on keratin isoform-specific functions, positioning recombinant K6HF as a tool to decode its unique contributions to epithelial biology and pathology. Ongoing research aims to clarify its physiological roles and explore clinical applications.
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