| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | JRK |
| Uniprot No | O75564 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-520aa |
| 活性数据 | MSTLYDIRAH KAQLLRFFAS SDSNKALEQR RTLHTPKLEH LDRVLYEWFL GKRSEGVPVS GPMLIEKAKD FYEQMQLTEP CVFSGGWLWR FKARHGIKKL DASSEKQSAD HQAAEQFCAF FRSLAAEHGL SAEQVYNADE TGLFWRCLPN PTPEGGAVPG PKQGKDRLTV LMCANATGSH RLKPLAIGKC SGPRAFKGIQ HLPVAYKAQG NAWVDKEIFS DWFHHIFVPS VREHFRTIGL PEDSKAVLLL DSSRAHPQEA ELVSSNVFTI FLPASVASLV QPMEQGIRRD FMRNFINPPV PLQGPHARYN MNDAIFSVAC AWNAVPSHVF RRAWRKLWPS VAFAEGSSSE EELEAECFPV KPHNKSFAHI LELVKEGSSC PGQLRQRQAA SWGVAGREAE GGRPPAATSP AEVVWSSEKT PKADQDGRGD PGEGEEVAWE QAAVAFDAVL RFAERQPCFS AQEVGQLRAL RAVFRSQQQV RRRRGALGAV VKVEALQEGP GGCGATAQSP LPCSSTAGDN |
| 分子量 | 61.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的关于重组人JRK蛋白的参考文献示例(仅供学术参考，实际文献需通过数据库查询)：
---
1. **文献名称**：*Cloning and Functional Characterization of Recombinant Human JRK Protein in Cell Proliferation*
**作者**：Chen L. et al.
**摘要**：本研究成功构建了人源JRK蛋白的重组表达载体，并在HEK293细胞中实现高效表达与纯化。通过体外实验证实，JRK蛋白具有ATP依赖性激酶活性，并调控细胞周期相关基因(如Cyclin D1)，提示其在肿瘤细胞增殖中可能发挥重要作用。
2. **文献名称**：*Crystal Structure Analysis of Recombinant JRK Reveals a Novel ATP-Binding Domain*
**作者**：Saito M. et al.
**摘要**：利用X射线晶体学解析了重组人JRK蛋白的三维结构，揭示了其独特的ATP结合域构象。分子动力学模拟显示该结构域与已知激酶家族存在差异，为设计靶向JRK的小分子抑制剂提供了结构基础。
3. **文献名称**：*Recombinant JRK Protein Attenuates Neurodegeneration in a Parkinson’s Disease Mouse Model*
**作者**：Gupta R. et al.
**摘要**：通过腹腔注射重组JRK蛋白至帕金森病模型小鼠，发现其显著减少多巴胺能神经元凋亡。进一步机制研究表明，JRK可能通过激活PI3K/AKT通路抑制氧化应激，提示其神经保护潜力。
4. **文献名称**：*High-Yield Production of Recombinant Human JRK in E. coli and Its Application in Drug Screening*
**作者**：Wang Y. et al.
**摘要**：开发了一种基于大肠杆菌的重组JRK蛋白高效表达系统，优化后的纯化策略使蛋白得率提升至20 mg/L。利用该蛋白建立高通量筛选平台，成功鉴定出3种抑制JRK酶活的先导化合物。
---
**注**：以上内容为模拟创作，JRK蛋白相关研究需查阅真实文献(建议使用PubMed/Google Scholar检索关键词：*recombinant human JRK protein* 或 *JRK gene function*)。若需真实文献，请提供更具体的蛋白功能背景或基因官方名称(如HUGO名称)。
Recombinant human JRK protein, also known as Jerky homolog or JRK/JRKL, is a genetically engineered version of a naturally occurring protein encoded by the JRK gene in humans. It belongs to the Jerky family of RNA-binding proteins, initially identified in Drosophila for its role in regulating neuronal excitability and seizure susceptibility. The human homolog shares structural and functional similarities, featuring conserved RNA-binding domains and a C-terminal region involved in protein-protein interactions.
JRK is implicated in post-transcriptional gene regulation, particularly in neurological processes. Studies suggest its involvement in mRNA stability, translation, and transport, impacting neuronal development and synaptic plasticity. Dysregulation of JRK has been tentatively linked to epilepsy and neurodevelopmental disorders, though mechanistic details remain under investigation. The recombinant form is typically expressed in E. coli or mammalian systems, often fused with tags (e.g., His-tag) for purification and detection.
Researchers utilize recombinant JRK protein to study its RNA-binding specificity, interactome mapping, and structural characterization. Its biochemical properties are explored to understand pathological mechanisms and potential therapeutic targets. Current challenges include clarifying its exact physiological RNA targets and regulatory networks in both healthy and diseased states, particularly in epilepsy models. The protein's conserved nature across species makes it valuable for translational neuroscience research.
×
