| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KATNAL1 |
| Uniprot No | Q9BW62 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-490aa |
| 活性数据 | MNLAEICDNA KKGREYALLG NYDSSMVYYQ GVMQQIQRHC QSVRDPAIKG KWQQVRQELL EEYEQVKSIV STLESFKIDK PPDFPVSCQD EPFRDPAVWP PPVPAEHRAP PQIRRPNREV RPLRKEMAGV GARGPVGRAH PISKSEKPST SRDKDYRARG RDDKGRKNMQ DGASDGEMPK FDGAGYDKDL VEALERDIVS RNPSIHWDDI ADLEEAKKLL REAVVLPMWM PDFFKGIRRP WKGVLMVGPP GTGKTMLAKA VATECGTTFF NVSSSTLTSK YRGESEKLVR LLFEMARFYA PTTIFIDEID SICSRRGTSD EHEASRRVKS ELLIQMDGVG GALENDDPSK MVMVLAATNF PWDIDEALRR RLEKRIYIPL PTAKGRAELL KINLREVELD PDIQLEDIAE KIEGYSGADI TNVCRDASLM AMRRRINGLS PEEIRALSKE ELQMPVTKGD FELALKKIAK SVSAADLEKY EKWMVEFGSA |
| 分子量 | 55.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KATNAL1蛋白的虚构参考文献示例，供参考(实际文献需通过学术数据库检索)：
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1. **"Recombinant Human KATNAL1 Expression and Microtubule-Severing Activity"**
*Smith A, et al.*
该研究在大肠杆菌中成功表达并纯化重组人KATNAL1蛋白，证实其具有ATP依赖的微管切割活性，并揭示了其酶活性受调控蛋白CRM-1的调控。
2. **"Structural Insights into KATNAL1's Role in Cilia Disassembly"**
*Chen B, et al.*
通过冷冻电镜解析了重组KATNAL1复合体的三维结构，阐明了其与微管相互作用的关键结构域，并证明其在纤毛动态拆卸中发挥核心作用。
3. **"KATNAL1 Knockout Models Reveal Impact on Spermatogenesis"**
*Tanaka K, et al.*
利用重组KATNAL1蛋白进行体外功能补偿实验，发现其缺失会导致精子细胞分裂异常，提示其在雄性生殖细胞发育中不可或缺。
4. **"CRISPR Screening Identifies KATNAL1 as a Regulator of Neuronal Morphogenesis"**
*Li X, et al.*
研究显示重组KATNAL1蛋白可恢复神经元突触微管网络的动态性，其表达水平异常与神经发育障碍中树突修剪缺陷直接相关。
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**注**：以上为模拟内容，实际研究需查询PubMed、Web of Science等平台，关键词如“recombinant KATNAL1”“katanin microtubule”等。
**Background of Recombinant Human KATNAL1 Protein**
KATNAL1 (Katanin Catalytic Subunit A1-Like 1) is a member of the AAA ATPase protein family, known for its microtubule-severing activity. It shares structural and functional homology with the catalytic subunit of katanin (p60), which regulates microtubule dynamics by disassembling microtubules in an ATP-dependent manner. KATNAL1 plays critical roles in cellular processes such as mitotic spindle formation, cilia disassembly, and spermatogenesis. Its activity is essential for maintaining microtubule network integrity, influencing cell division, motility, and intracellular transport.
Studies link KATNAL1 dysfunction to reproductive disorders and ciliopathies. For instance, mutations or dysregulation of KATNAL1 are implicated in male infertility due to its role in sperm flagellum formation and cilia-dependent signaling. Recombinant human KATNAL1 protein, typically produced in systems like *E. coli* or mammalian cell lines, enables mechanistic studies of its enzymatic properties, substrate interactions, and regulatory pathways. Recent structural analyses reveal that KATNAL1 assembles into hexameric complexes, leveraging ATP hydrolysis to induce conformational changes that disrupt microtubule lattices.
Research on recombinant KATNAL1 also explores its therapeutic potential, particularly in diseases linked to microtubule dysregulation. Its study advances understanding of cellular architecture, developmental biology, and pathogenic mechanisms underlying cilia-related disorders.
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