| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IVL |
| Uniprot No | P07476 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-585aa |
| 活性数据 | MSQQHTLPVT LSPALSQELL KTVPPPVNTH QEQMKQPTPL PPPCQKVPVE LPVEVPSKQE EKHMTAVKGL PEQECEQQQK EPQEQELQQQ HWEQHEEYQK AENPEQQLKQ EKTQRDQQLN KQLEEEKKLL DQQLDQELVK RDEQLGMKKE QLLELPEQQE GHLKHLEQQE GQLKHPEQQE GQLELPEQQE GQLELPEQQE GQLELPEQQE GQLELPEQQE GQLELPEQQE GQLELPQQQE GQLELSEQQE GQLELSEQQE GQLKHLEHQE GQLEVPEEQM GQLKYLEQQE GQLKHLDQQE KQPELPEQQM GQLKHLEQQE GQPKHLEQQE GQLEQLEEQE GQLKHLEQQE GQLEHLEHQE GQLGLPEQQV LQLKQLEKQQ GQPKHLEEEE GQLKHLVQQE GQLKHLVQQE GQLEQQERQV EHLEQQVGQL KHLEEQEGQL KHLEQQQGQL EVPEQQVGQP KNLEQEEKQL ELPEQQEGQV KHLEKQEAQL ELPEQQVGQP KHLEQQEKHL EHPEQQDGQL KHLEQQEGQL KDLEQQKGQL EQPVFAPAPG QVQDIQPALP TKGEVLLPVE HQQQKQEVQW PPKHK |
| 分子量 | 68.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IVL(involucrin)蛋白的参考文献示例(请注意，这些文献为模拟内容，实际引用时建议通过学术数据库核实)：
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1. **文献名称**: *"Recombinant Human Involucrin Production in Bacterial Systems: Structural and Functional Analysis"*
**作者**: Zhang, L., Wang, Y., & Chen, H.
**摘要**: 本研究利用大肠杆菌表达系统成功重组表达人IVL蛋白，并通过质谱和圆二色谱验证其结构与天然蛋白的一致性，证实重组IVL在体外能够参与角质细胞膜交联反应。
2. **文献名称**: *"Involucrin-Mediated Skin Barrier Repair: A Study Using Recombinant Protein Delivery"*
**作者**: Tanaka, K., et al.
**摘要**: 通过脂质体递送重组人IVL蛋白至受损表皮模型，研究发现其能加速皮肤屏障功能的恢复，并显著减少水分流失，为治疗皮炎提供潜在策略。
3. **文献名称**: *"Role of Recombinant Involucrin in Epidermal Differentiation and Its Interaction with Keratin Filaments"*
**作者**: Müller, S., & Novak, J.
**摘要**: 研究重组IVL在三维表皮培养模型中的作用，发现其能够促进角质层分化和细胞骨架重组，并增强与角蛋白丝的相互作用，揭示了其在表皮终末分化中的分子机制。
4. **文献名称**: *"High-Yield Purification and Biocompatibility Assessment of Recombinant Human IVL for Clinical Applications"*
**作者**: Gupta, R., et al.
**摘要**: 开发了一种高效纯化重组人IVL的工艺，并通过细胞毒性实验证明其良好的生物相容性，为未来用于皮肤再生医学奠定基础。
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**备注**：上述文献为示例性质，具体研究需通过PubMed、ScienceDirect等数据库检索关键词(如"recombinant human involucrin")获取真实文献。早期经典研究(如Simon和Green的论文)可能更多涉及天然IVL功能，而重组蛋白的应用常见于近年研究。
**Background of Recombinant Human IVL Protein**
Involucrin (IVL) is a key structural protein in human epidermal differentiation, primarily expressed in the suprabasal layers of stratified squamous epithelia, such as the skin. It plays a critical role in forming the cornified envelope (CE), a resilient barrier that protects against environmental stressors, prevents water loss, and maintains tissue integrity. IVL serves as a scaffold during CE assembly, crosslinking with other proteins (e.g., loricrin, filaggrin) and lipids via transglutaminase-mediated bonds. Its expression is tightly regulated during keratinocyte differentiation, making it a biomarker for terminal epidermal maturation.
Dysregulation of IVL is linked to skin disorders, including psoriasis, eczema, and ichthyosis, as well as cancers like squamous cell carcinoma. Recombinant human IVL (rhIVL) is produced using genetic engineering techniques (e.g., bacterial or mammalian expression systems) to study its biological functions, interactions, and therapeutic potential. Applications include modeling skin diseases, screening barrier-enhancing therapies, and developing regenerative strategies. Additionally, rhIVL aids in elucidating molecular mechanisms of CE formation and testing drug delivery systems targeting epidermal barriers. Its recombinant form ensures consistent quality and scalability, facilitating translational research in dermatology and beyond.
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