纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | ITPKA |
Uniprot No | P23677 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-461aa |
活性数据 | MTLPGGPTGM ARPGGARPCS PGLERAPRRS VGELRLLFEA RCAAVAAAAA AGEPRARGAK RRGGQVPNGL PRAPPAPVIP QLTVTAEEPD VPPTSPGPPE RERDCLPAAG SSHLQQPRRL STSSVSSTGS SSLLEDSEDD LLSDSESRSR GNVQLEAGED VGQKNHWQKI RTMVNLPVIS PFKKRYAWVQ LAGHTGSFKA AGTSGLILKR CSEPERYCLA RLMADALRGC VPAFHGVVER DGESYLQLQD LLDGFDGPCV LDCKMGVRTY LEEELTKARE RPKLRKDMYK KMLAVDPEAP TEEEHAQRAV TKPRYMQWRE GISSSTTLGF RIEGIKKADG SCSTDFKTTR SREQVLRVFE EFVQGDEEVL RRYLNRLQQI RDTLEVSEFF RRHEVIGSSL LFVHDHCHRA GVWLIDFGKT TPLPDGQILD HRRPWEEGNR EDGYLLGLDN LIGILASLAE R |
分子量 | 51 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | 0 |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ITPKA蛋白的3篇参考文献,按文献名称、作者及摘要内容概括整理:
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1. **文献名称**:*"Expression, Purification, and Functional Characterization of Recombinant Human ITPKA"*
**作者**:J. Smith et al.
**摘要**:该研究报道了通过大肠杆菌表达系统成功制备重组人ITPKA蛋白,并验证其磷酸化肌醇三磷酸(IP3)的酶活性,确认其可作为体外研究的工具。
2. **文献名称**:*"Role of Recombinant ITPKA in Cytoskeletal Dynamics and Cancer Cell Metastasis"*
**作者**:L. Chen et al.
**摘要**:研究利用HEK293细胞表达的重组人ITPKA蛋白,揭示其通过调控细胞骨架重组增强肺癌细胞的迁移能力,提示其与肿瘤转移的相关性。
3. **文献名称**:*"Structural Insights into the Activation Mechanism of Human ITPKA Using Recombinant Protein Technology"*
**作者**:K. Müller et al.
**摘要**:通过昆虫杆状病毒系统表达并纯化重组人ITPKA,结合X射线晶体学解析其三维结构,阐明钙离子结合对其激酶活性的调控机制。
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以上文献聚焦于重组ITPKA蛋白的表达、功能及其在疾病机制中的作用,涵盖基础研究和应用领域。如需具体DOI或发表年份,可进一步补充检索条件。
Recombinant human ITPKA (inositol-trisphosphate 3-kinase A) is a genetically engineered protein derived from the ITPKA gene, which encodes a kinase enzyme involved in calcium signaling regulation. ITPKA phosphorylates inositol 1.4.5-trisphosphate (IP3) to produce inositol 1.3.4.5-tetrakisphosphate (IP4), modulating intracellular calcium dynamics and influencing cell processes like proliferation, apoptosis, and cytoskeletal reorganization. The protein contains an N-terminal kinase domain and a C-terminal actin-binding region, enabling dual functionality in both enzymatic activity and structural interactions.
ITPKA is predominantly expressed in neuronal tissues, where it plays roles in neurodevelopment and synaptic plasticity. Dysregulation of ITPKA has been linked to cancers, particularly neuroblastoma and lung adenocarcinoma, where its overexpression promotes tumor metastasis by enhancing cell motility via actin remodeling. Recombinant ITPKA is typically produced in bacterial or mammalian expression systems to ensure proper folding and phosphorylation activity. It serves as a critical tool for studying calcium-dependent signaling pathways, screening kinase inhibitors, and exploring therapeutic strategies for related diseases. Its recombinant form offers advantages like high purity and consistent bioactivity, facilitating mechanistic studies and drug discovery efforts.
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