| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITGB4BP |
| Uniprot No | P56537 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-245aa |
| 活性数据 | MAVRASFENNCEIGCFAKLTNTYCLVAIGGSENFYSVFEGELSDTIPVVHASIAGCRIIGRMCVGNRHGLLVPNNTTDQELQHIRNSLPDTVQIRRVEERLSALGNVTTCNDYVALVHPDLDRETEEILADVLKVEVFRQTVADQVLVGSYCVFSNQGGLVHPKTSIEDQDELSSLLQVPLVAGTVNRGSEVIAAGMVVNDWCAFCGLDTTSTELSVVESVFKLNEAQPSTIATSMRDSLIDSLT |
| 分子量 | 53 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ITGB4BP(整合素β4结合蛋白)的虚构参考文献示例(注：文献名称及作者均为假设，仅供参考)：
1. **文献名称**：*Structural Insights into Recombinant Human ITGB4BP and Its Role in Cell Adhesion*
**作者**：Zhang et al. (2021)
**摘要**：研究通过X射线晶体学解析了重组人ITGB4BP的三维结构，发现其通过特定结构域与整合素β4亚基结合，调控肿瘤细胞的黏附和迁移。
2. **文献名称**：*ITGB4BP Recombinant Protein Attenuates Fibrosis in a Mouse Model of Lung Injury*
**作者**：Wang et al. (2020)
**摘要**：实验表明，重组人ITGB4BP通过抑制TGF-β信号通路显著减轻了小鼠肺纤维化，提示其作为抗纤维化治疗靶点的潜力。
3. **文献名称**：*Functional Characterization of Recombinant ITGB4BP in Cancer Stem Cell Maintenance*
**作者**：Li et al. (2019)
**摘要**：体外研究证明，重组人ITGB4BP通过调控Wnt/β-catenin通路促进癌症干细胞自我更新，为靶向肿瘤干细胞提供依据。
4. **文献名称**：*High-Yield Expression and Purification of Recombinant Human ITGB4BP in E. coli*
**作者**：Chen et al. (2022)
**摘要**：开发了一种高效的大肠杆菌表达系统，优化重组人ITGB4BP的可溶性表达及纯化工艺，为大规模生产奠定基础。
**注意**：以上文献为示例文本，实际研究中请通过PubMed、Google Scholar等数据库检索真实文献。
Recombinant human ITGB4BP (Integrin Beta-4-Binding Protein), also known as EIF6 or B4BP, is a conserved multifunctional protein involved in ribosomal maturation, cell adhesion, and signal transduction. Structurally, ITGB4BP contains a conserved N-terminal thioredoxin-like domain and a C-terminal charged region. It interacts directly with the cytoplasmic tail of integrin β4. a key component of hemidesmosomes, to regulate epithelial cell adhesion, migration, and mechanotransduction.
Originally identified as a translational regulator (eIF6), it plays dual roles in ribosome biogenesis and integrin-mediated extracellular signaling. Dysregulation of ITGB4BP is linked to cancer progression (e.g., metastasis, chemoresistance) and genetic disorders like epidermolysis bullosa, where impaired hemidesmosome function causes skin fragility. Its overexpression is observed in colorectal, breast, and pancreatic cancers, correlating with poor prognosis.
Recombinant ITGB4BP is produced in systems like E. coli or mammalian cells for functional studies, enabling research into ribosome-ECM crosstalk and therapeutic targeting. Applications include investigating cell-matrix interactions, tumor microenvironment adaptation, and developing inhibitors for precision oncology. Current studies focus on its role in coordinating protein synthesis with mechanical signaling during tissue remodeling and disease.
×
