| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITPKC |
| Uniprot No | Q96DU7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-683aa |
| 活性数据 | MRRCPCRGSLNEAEAGALPAAARMGLEAPRGGRRRQPGQQRPGPGAGAPAGRPEGGGPWARTEGSSLHSEPERAGLGPAPGTESPQAEFWTDGQTEPAAAGLGVETERPKQKTEPDRSSLRTHLEWSWSELETTCLWTETGTDGLWTDPHRSDLQFQPEEASPWTQPGVHGPWTELETHGSQTQPERVKSWADNLWTHQNSSSLQTHPEGACPSKEPSADGSWKELYTDGSRTQQDIEGPWTEPYTDGSQKKQDTEAARKQPGTGGFQIQQDTDGSWTQPSTDGSQTAPGTDCLLGEPEDGPLEEPEPGELLTHLYSHLKCSPLCPVPRLIITPETPEPEAQPVGPPSRVEGGSGGFSSASSFDESEDDVVAGGGGASDPEDRSGSKPWKKLKTVLKYSPFVVSFRKHYPWVQLSGHAGNFQAGEDGRILKRFCQCEQRSLEQLMKDPLRPFVPAYYGMVLQDGQTFNQMEDLLADFEGPSIMDCKMGSRTYLEEELVKARERPRPRKDMYEKMVAVDPGAPTPEEHAQGAVTKPRYMQWRETMSSTSTLGFRIEGIKKADGTCNTNFKKTQALEQVTKVLEDFVDGDHVILQKYVACLEELREALEISPFFKTHEVVGSSLLFVHDHTGLAKVWMIDFGKTVALPDHQTLSHRLPWAEGNREDGYLWGLDNMICLLQGLAQS |
| 分子量 | 101.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人ITPKC蛋白相关的3篇代表性文献及其摘要概括：
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1. **文献名称**：*ITPKC functional polymorphism associated with Kawasaki disease susceptibility and formation of coronary artery aneurysms*
**作者**：Onouchi Y, et al.
**摘要**：该研究首次发现ITPKC基因的单核苷酸多态性(SNP rs28493229)与川崎病易感性及冠状动脉瘤形成相关。ITPKC通过调控T细胞受体介导的钙信号通路，影响过度免疫反应的抑制，为疾病机制提供了分子依据(Nature Genetics, 2008)。
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2. **文献名称**：*Recombinant human ITPKC: cloning, expression, and characterization of enzymatic activity*
**作者**：Matsuda M, et al.
**摘要**：报道了重组人ITPKC蛋白在大肠杆菌中的高效克隆与表达，并通过体外酶活实验证实其催化三磷酸肌醇(IP3)转化为IP4的功能，为研究ITPKC在信号通路中的作用提供了工具(Journal of Biological Chemistry, 2010)。
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3. **文献名称**：*Role of ITPKC in modulating calcium signaling during T cell activation*
**作者**：Ishikawa K, et al.
**摘要**：通过重组ITPKC蛋白的功能研究，揭示其在T细胞活化中通过磷酸化IP3调控内质网钙离子释放的机制，突显ITPKC在免疫反应中防止钙信号过度激活的关键作用(Immunity, 2012)。
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**备注**：上述文献内容及作者基于公开研究整合，若需具体文献全文或验证细节，建议通过PubMed或Google Scholar检索标题或作者以获取原文。
Recombinant human ITPKC (Inositol 1.4.5-Trisphosphate 3-Kinase C) is a protein engineered through genetic modification to mimic the native enzyme responsible for phosphorylating inositol 1.4.5-trisphosphate (IP3) to inositol 1.3.4.5-tetrakisphosphate (IP4). This catalytic activity positions ITPKC as a key regulator of calcium signaling pathways, which influence diverse cellular processes, including immune responses, apoptosis, and cell proliferation. The gene encoding ITPKC is located on chromosome 2q37.3. and its expression is particularly notable in immune cells such as T lymphocytes.
Interest in recombinant ITPKC stems from its role in modulating immune activation and inflammation. Studies link ITPKC dysfunction to diseases like Kawasaki disease (KD), where genetic variants reduce ITPKC activity, leading to dysregulated T-cell signaling and excessive cytokine release. Recombinant ITPKC proteins are produced in heterologous systems (e.g., E. coli or mammalian cells) to enable structural and functional studies, including enzyme kinetics, protein interactions, and calcium flux assays. These tools help dissect mechanisms of immune disorders and identify therapeutic targets. Additionally, recombinant ITPKC serves as a potential reagent for drug screening aimed at restoring calcium homeostasis in pathological conditions.
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