| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ISY1 |
| Uniprot No | Q9ULR0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-285aa |
| 活性数据 | MARNAEKAMTALARFRQAQLEEGKVKERRPFLASECTELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPLEQEYEKKLRAELVEKWKAEREARLARGEKEEEEEEEEEINIYAVTEEESDEEGSQEKGGDDSQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEARRLLGY |
| 分子量 | 59.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ISY1蛋白的3篇参考文献(基于模拟数据生成，实际引用需核实原文)：
1. **文献名称**：*ISY1 regulates mitochondrial DNA excision during cellular reprogramming*
**作者**：Smith J, et al.
**摘要**：研究利用重组人ISY1蛋白揭示其在维持线粒体基因组稳定性中的作用，通过体外实验证明ISY1通过调控RNA剪接复合体参与线粒体DNA损伤修复。
2. **文献名称**：*Recombinant human ISY1 protein facilitates spliceosome assembly in vitro*
**作者**：Li H, Wang Y, et al.
**摘要**：通过大肠杆菌系统表达并纯化重组人ISY1蛋白，发现其能够增强剪接体前体复合物的形成，为RNA剪接机制研究提供工具。
3. **文献名称**：*Structural and functional analysis of the ISY1 protein in mRNA processing*
**作者**：Kumar R, et al.
**摘要**：利用昆虫细胞表达系统制备重组ISY1蛋白，结合X射线晶体学分析其三维结构，阐明其通过锌指结构域与RNA结合的功能基础。
4. **文献名称**：*ISY1 deficiency disrupts neuronal differentiation via aberrant splicing*
**作者**：Chen L, et al.
**摘要**：在HEK293细胞中表达重组ISY1蛋白，证实其在神经分化过程中通过调控特定mRNA剪接事件影响神经元成熟，为神经系统疾病研究提供线索。
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**备注**：以上内容为模拟生成，实际文献需通过PubMed、Google Scholar等平台检索关键词“recombinant human ISY1”或“ISY1 protein function”获取。
Recombinant human ISY1 (rhISY1) protein is a genetically engineered version of the ISY1 (Inhibitor of Sterile Y-motif 1) protein, encoded by the *ISY1* gene in humans. ISY1 is a RNA-binding protein implicated in pre-mRNA splicing and post-transcriptional gene regulation. It interacts with spliceosome components, influencing splice site selection and alternative splicing events critical for diverse cellular processes, including cell cycle control, apoptosis, and differentiation. Studies suggest ISY1 plays roles in maintaining genomic stability and regulating gene expression in response to cellular stress. Recombinant ISY1 is typically produced in *E. coli* or mammalian expression systems, enabling functional studies of its structure, RNA-binding domains, and interactions with spliceosomal complexes. Its recombinant form facilitates research into splicing mechanisms, disease-associated splicing errors (e.g., in cancer or neurodegeneration), and potential therapeutic targeting. Purified rhISY1 is also used to develop assays for drug screening or to study mutations affecting splicing fidelity. Ongoing research aims to clarify ISY1’s regulatory networks and its crosstalk with other RNA-processing factors, offering insights into its broader roles in cellular homeostasis and disease.
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