| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IRXL1 |
| Uniprot No | Q8IYA7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-352aa |
| 活性数据 | MNTIVFNKLSSAVLFEDGGASERERGGRPYSGVLDSPHARPEVGIPDGPPLKDNLGLRHRRTGARQNGGKVRHKRQALQDMARPLKQWLYKHRDNPYPTKTEKILLALGSQMTLVQVSNWFANARRRLKNTVRQPDLSWALRIKLYNKYVQGNAERLSVSSDDSCSEDGENPPRTHMNEGGYNTPVHHPVIKSENSVIKAGVRPESRASEDYVAPPKYKSSLLNRYLNDSLRHVMATNTTMMGKTRQRNHSGSFSSNEFEEELVSPSSSETEGNFVYRTDTLENGSNKGESAANRKGPSKDDTYWKEINAAMALTNLAQGKDKLQGTTSCIIQKSSHIAEVKTVKVPLVQQF |
| 分子量 | 65.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IRXL1蛋白的虚构参考文献示例，供参考：
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1. **文献名称**: *Expression and functional analysis of recombinant human IRXL1 in developmental signaling pathways*
**作者**: Zhang L., et al.
**摘要**: 本研究在大肠杆菌系统中成功表达并纯化了重组人IRXL1蛋白，证实其在Wnt/β-catenin信号通路中的调控作用，并发现其通过结合SMAD4影响胚胎干细胞分化。
2. **文献名称**: *Structural characterization of IRXL1 reveals its role in neural crest cell migration*
**作者**: Tanaka K., et al.
**摘要**: 通过X射线晶体学解析IRXL1蛋白的三维结构，发现其C端结构域对神经嵴细胞迁移至关重要，并证明重组IRXL1可抑制斑马鱼模型中的异常细胞迁移。
3. **文献名称**: *IRXL1 recombinant protein suppresses tumor growth in colorectal cancer models*
**作者**: Gupta R., et al.
**摘要**: 在昆虫细胞中表达的重组IRXL1蛋白显著抑制结直肠癌细胞增殖，机制涉及调控Notch通路关键因子Hes1的表达，为潜在抗癌靶点提供依据。
4. **文献名称**: *Role of IRXL1 in cardiac development: Insights from recombinant protein rescue experiments*
**作者**: Müller S., et al.
**摘要**: 利用HEK293细胞表达的重组IRXL1蛋白修复小鼠心脏发育缺陷模型，证明其通过调控TGF-β信号维持心肌细胞分化和心脏形态发生。
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注：以上文献为模拟示例，实际研究中请通过学术数据库验证。
Recombinant human IRXL1 (Iroquois-class homeoprotein-like 1) is a engineered protein derived from the IRX gene family, which encodes evolutionarily conserved homeodomain transcription factors. The IRX family plays critical roles in embryonic development, particularly in patterning tissues like the nervous system, heart, and limbs. IRXL1 shares structural homology with other IRX proteins, characterized by a conserved homeodomain responsible for DNA binding and a conserved downstream TALE motif. While its exact biological functions remain less characterized compared to other IRX members (e.g., IRX3 or IRX5), studies suggest involvement in cellular differentiation, organogenesis, and regulation of developmental signaling pathways such as BMP and Wnt.
The recombinant form is typically produced using heterologous expression systems (e.g., E. coli or mammalian cell lines) to enable functional studies. It often includes affinity tags (e.g., His-tag) for purification and detection. Research focuses on elucidating IRXL1's role in transcriptional regulation, protein-protein interactions, and its potential link to developmental disorders or diseases. Notably, aberrant expression of IRX genes has been implicated in cancers and congenital anomalies, positioning recombinant IRXL1 as a tool to explore molecular mechanisms or therapeutic targets. Current challenges include understanding post-translational modifications and tissue-specific isoforms, which recombinant proteins help address by providing standardized, high-purity samples for assays.
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