| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ISOC1 |
| Uniprot No | Q96CN7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-298aa |
| 活性数据 | MAAAEPAVLALPNSGAGGAGAPSGTVPVLFCFSVFARPSSVPHGAGYELLIQKFLSLYGDQIDMHRKFVVQLFAEEWGQYVDLPKGFAVSERCKVRLVPLQIQLTTLGNLTPSSTVFFCCDMQERFRPAIKYFGDIISVGQRLLQGARILGIPVIVTEQYPKGLGSTVQEIDLTGVKLVLPKTKFSMVLPEVEAALAEIPGVRSVVLFGVETHVCIQQTALELVGRGVEVHIVADATSSRSMMDRMFALERLARTGIIVTTSEAVLLQLVADKDHPKFKEIQNLIKASAPESGLLSKV |
| 分子量 | 59.18 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ISOC1蛋白的假设性参考文献示例(仅供格式参考，实际文献需通过学术数据库查询)：
1. **文献名称**: *Cloning and Expression of Recombinant Human ISOC1 in Escherichia coli*
**作者**: Zhang Y, et al.
**摘要**: 研究描述了人ISOC1基因的克隆策略，通过大肠杆菌表达系统实现蛋白可溶性表达，并利用亲和层析技术纯化，验证了重组蛋白的体外稳定性。
2. **文献名称**: *Structural Characterization of ISOC1 and Its Role in Mitochondrial Metabolism*
**作者**: Lee S, et al.
**摘要**: 采用X射线晶体学解析ISOC1蛋白结构，发现其与线粒体肽酶复合物相互作用，可能参与调控细胞能量代谢通路。
3. **文献名称**: *ISOC1 Knockdown Impairs Cell Cycle Progression in Cancer Cells*
**作者**: Gupta R, et al.
**摘要**: 通过重组ISOC1蛋白功能实验，发现其缺失导致肿瘤细胞周期停滞，暗示其在细胞增殖中的潜在作用。
4. **文献名称**: *Functional Analysis of ISOC1 Mutations in Genetic Disorders*
**作者**: Müller J, et al.
**摘要**: 研究筛选了ISOC1基因突变，利用重组蛋白模型揭示突变对酶活性的影响，关联其与特定代谢疾病的病理机制。
**提示**：上述为示例，实际研究中可检索**PubMed**或**Google Scholar**，关键词“recombinant human ISOC1”或“ISOC1 protein function”获取真实文献。
Recombinant human ISOC1 (ISOCHORISMATASE domain-containing protein 1), also known as C7orf28. is a protein encoded by the ISOC1 gene located on chromosome 7. Its primary structure includes an isochorismatase-like domain, suggesting potential enzymatic activity related to isomerase or hydrolase functions, though its precise biochemical role remains under investigation. ISOC1 is ubiquitously expressed in human tissues, particularly in the brain, liver, and kidneys, and localizes to mitochondria, implying involvement in mitochondrial metabolism or stress responses. Studies link ISOC1 to cellular processes such as proliferation, apoptosis, and immune regulation. Dysregulation of ISOC1 has been associated with neurodegenerative diseases, cancers, and autoimmune disorders, making it a subject of interest in biomarker and therapeutic research. Recombinant ISOC1 protein is typically produced using bacterial or mammalian expression systems, enabling functional studies to elucidate its molecular interactions, structural properties, and role in disease pathways. Its application spans enzyme activity assays, protein-protein interaction studies, and antibody development. Despite progress, the full mechanistic landscape of ISOC1. including its endogenous substrates and regulatory networks, requires further exploration to harness its potential in biomedical applications.
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