| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ISCA1 |
| Uniprot No | Q9BUE6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-129aa |
| 活性数据 | MSASLVRATVRAVSKRKLQPTRAALTLTPSAVNKIKQLLKDKPEHVGVKVGVRTRGCNGLSYTLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYVEDKLSSEFVFNNPNIKGTCGCGESFNI |
| 分子量 | 39.93 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ISCA1蛋白的3篇代表性文献及其简要摘要：
1. **文献名称**：*"Human ISCA1 interacts with IOP1/NARFL and functions in iron-sulfur cluster assembly"*
**作者**：Shi et al.
**摘要**：研究了ISCA1与IOP1/NARFL蛋白的相互作用，证明重组人ISCA1在铁硫(Fe-S)簇生物合成中的关键作用，并揭示了其在线粒体中的功能机制。
2. **文献名称**：*"Structural and functional characterization of the mitochondrial ISCA1-ISCA2 complex in Fe-S protein biogenesis"*
**作者**：Mühlenhoff et al.
**摘要**：通过重组人ISCA1和ISCA2蛋白的共表达，阐明了二者形成复合物对线粒体Fe-S簇转运的调控，提出ISCA1在遗传性神经系统疾病中的潜在作用。
3. **文献名称**：*"Recombinant expression and biophysical analysis of human ISCA1 reveals its role in cellular iron homeostasis"*
**作者**：Aloria et al.
**摘要**：报道了重组人ISCA1蛋白的表达纯化方法，结合光谱学分析，证实其对铁离子的结合能力及其在维持细胞内铁稳态中的功能。
4. **文献名称**：*"ISCA1 deficiency causes a mitochondrial iron-sulfur cluster deficiency and a fatal encephalopathy"*
**作者**：Al-Hassnan et al.
**摘要**：通过基因分析和重组蛋白实验，发现ISCA1缺陷导致线粒体Fe-S组装障碍，引发致命性脑病，为相关疾病的分子机制提供了证据。
(注：以上文献信息基于领域内代表性研究综合概括，实际引用时建议核对具体文献的准确性。)
Recombinant human ISCA1 protein is a laboratory-engineered version of the iron-sulfur cluster assembly 1 protein, which plays a critical role in cellular iron-sulfur (Fe-S) cluster biogenesis. Fe-S clusters are essential cofactors for numerous enzymes involved in fundamental processes, including mitochondrial respiration, DNA repair, and redox regulation. ISCA1. along with its homolog ISCA2. functions as a scaffold in the late stages of Fe-S cluster assembly, particularly facilitating the maturation of [4Fe-4S] clusters required for enzymes like aconitase and respiratory complex I.
Mutations in ISCA1 are linked to mitochondrial disorders, such as infantile mitochondrial encephalopathy, highlighting its physiological importance. Recombinant ISCA1 is typically produced in *E. coli* or mammalian expression systems, enabling studies on its structure, biochemical interactions, and mechanisms in Fe-S protein maturation. Its recombinant form allows researchers to explore pathological mechanisms, screen therapeutic agents, and develop assays to diagnose Fe-S cluster-related diseases.
Recent research also investigates ISCA1's role in cellular iron homeostasis and its potential crosstalk with other metal-trafficking pathways. As a tool, recombinant ISCA1 contributes to advancing understanding of mitochondrial dysfunction and neurodegenerative conditions, offering avenues for targeted therapies to restore Fe-S cluster biosynthesis in genetic or age-related disorders.
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