| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IRX5 |
| Uniprot No | P78411 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 204-264aa |
| 活性数据 | PQKPEDKADPEGPEAGGAEQKAASGCERLQGPPTPAGKETEGSLSDSDFKEPPSEGRLDAL |
| 分子量 | 32.45 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IRX5蛋白的参考文献示例(注：以下为示例性内容，建议通过学术数据库核实具体文献):
1. **"Functional characterization of recombinant human IRX5 in cardiac development"**
- **作者**: Zhang Y. et al.
- **摘要**: 研究报道了通过哺乳动物细胞系统表达重组人IRX5蛋白，发现其通过调控TGF-β信号通路参与心肌细胞分化和心脏发育，为先天性心脏病的机制提供了新见解。
2. **"Expression and DNA-binding properties of IRX5 homeodomain protein"**
- **作者**: Smith J.R. et al.
- **摘要**: 利用大肠杆菌成功表达并纯化重组IRX5蛋白，通过电泳迁移实验(EMSA)证明其特异性结合心脏发育相关基因的启动子区域，揭示了其转录调控功能。
3. **"IRX5 promotes melanoma progression via Wnt/β-catenin signaling"**
- **作者**: Lee S. et al.
- **摘要**: 研究使用重组人IRX5蛋白处理黑色素瘤细胞，发现其通过激活Wnt通路增强肿瘤侵袭性，表明IRX5可能成为癌症治疗的新靶点。
4. **"Structural analysis of IRX5 homeobox domain by X-ray crystallography"**
- **作者**: Kumar A. et al.
- **摘要**: 通过重组表达和纯化IRX5蛋白的DNA结合结构域，解析其晶体结构，揭示了其与靶基因相互作用的分子基础。
建议通过 **PubMed** 或 **Google Scholar** 以关键词 "recombinant human IRX5 protein" 或 "IRX5 overexpression" 检索最新文献。
Recombinant human IRX5 protein is a engineered form of the Iroquois-class homeodomain transcription factor IRX5 (Iroquois homeobox protein 5), which plays critical roles in developmental processes and tissue homeostasis. The IRX gene family, conserved across vertebrates, is involved in patterning during embryogenesis, particularly in the heart, nervous system, and limb development. IRX5 is notably implicated in cardiac electrophysiology, where it regulates the transcription of potassium channels like KCNIP2. influencing ventricular repolarization. Dysregulation of IRX5 has been associated with arrhythmias and pathological cardiac remodeling. Additionally, IRX5 shows context-dependent roles in cancer, acting as either a tumor suppressor or promoter in malignancies such as glioblastoma and breast cancer.
The recombinant version is typically produced using expression systems (e.g., E. coli, mammalian cells) with tags (e.g., His-tag) for purification. This enables functional studies to dissect IRX5's interaction networks, DNA-binding specificity, and regulatory mechanisms. Researchers employ it to explore its dual role in development and disease, including its crosstalk with signaling pathways like Wnt/β-catenin. Its therapeutic potential is being investigated for cardiac disorders and precision oncology. However, challenges remain in understanding tissue-specific isoforms and post-translational modifications that modulate its activity. As a standardized reagent, recombinant IRX5 accelerates biomedical research bridging developmental biology and pathophysiology.
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