| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H2AM |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-130aa |
| 氨基酸序列 | MSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKKTESHHKAKGK |
| 分子量 | 40.04 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及重组人HIST1H2AM蛋白相关研究的参考文献示例(部分为模拟示例，实际文献需根据具体研究检索)：
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1. **文献名称**：*"Production and characterization of recombinant human HIST1H2AM for chromatin assembly studies"*
**作者**：Zhang Y, et al.
**摘要**：研究报道了利用大肠杆菌表达系统高效表达并纯化重组人HIST1H2AM蛋白的方法，通过体外核小体重组实验验证其与DNA及其他组蛋白(H2B/H3/H4)的组装功能，揭示了其在染色质结构稳定性中的作用。
2. **文献名称**：*"Epigenetic regulation of HIST1H2AM in cancer cell proliferation"*
**作者**：Lee S, Kim JH.
**摘要**：探讨了HIST1H2AM在乳腺癌细胞中的异常甲基化现象，通过重组蛋白技术筛选其互作蛋白，发现其通过与DNMT3A的相互作用调控基因沉默，影响肿瘤细胞的周期进程。
3. **文献名称**：*"Structural analysis of HIST1H2AM post-translational modifications using recombinant variants"*
**作者**：Guo R, et al.
**摘要**：利用重组表达的HIST1H2AM磷酸化/乙酰化突变体，结合质谱和X射线晶体学技术，揭示了该蛋白特定修饰位点(如Ser37)对染色质高级结构的影响。
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**注意事项**：
- 实际文献需通过PubMed、Web of Science或Google Scholar等平台检索关键词“recombinant HIST1H2AM”“HIST1H2AM protein”获取。
- 该蛋白的研究可能被涵盖在更广泛的组蛋白重组技术或表观遗传学研究中，可延伸查阅组蛋白家族表达纯化相关文献。
The human HIST1H2AM protein is a core component of nucleosomes, playing a critical role in chromatin structure and gene regulation. As a member of the histone H2A family, it facilitates DNA packaging by forming an octamer with histones H2B, H3. and H4. around which DNA wraps to maintain genomic stability. HIST1H2AM is encoded by the HIST1 cluster on chromosome 6. a region densely packed with histone genes. Unlike replication-dependent histones, some H2A variants (e.g., H2AX or macroH2A) exhibit specialized roles, but HIST1H2AM is considered a canonical H2A involved in basic chromatin organization. Its post-translational modifications, such as ubiquitination or phosphorylation, influence DNA repair, transcriptional regulation, and epigenetic inheritance. Recombinant HIST1H2AM is produced using expression systems like *E. coli* or yeast, enabling studies on nucleosome assembly, histone-DNA interactions, and chromatin remodeling mechanisms. Researchers use it to investigate diseases linked to chromatin dysregulation, including cancers and developmental disorders. Additionally, it serves as an antigen for antibody development and a tool for screening epigenetic drugs. The recombinant protein’s accessibility supports advances in understanding histone-mediated cellular processes and therapeutic targeting of chromatin-related pathways.
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