| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIRIP3 |
| Uniprot No | Q9BW71 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-556aa |
| 氨基酸序列 | MAREKEMQEFTRSFFRGRPDLSTLTHSIVRRRYLAHSGRSHLEPEEKQALKRLVEEELLKMQVDEAASREDKLDLTKKGKRPPTPCSDPERKRFRFNSESESGSEASSPDYFGPPAKNGVAAEVSPAKEENPRRASKAVEESSDEERQRDLPAQRGEESSEEEEKGYKGKTRKKPVVKKQAPGKASVSRKQAREESEESEAEPVQRTAKKVEGNKGTKSLKESEQESEEEILAQKKEQREEEVEEEEKEEDEEKGDWKPRTRSNGRRKSAREERSCKQKSQAKRLLGDSDSEEEQKEAASSGDDSGRDREPPVQRKSEDRTQLKGGKRLSGSSEDEEDSGKGEPTAKGSRKMARLGSTSGEESDLEREVSDSEAGGGPQGERKNRSSKKSSRKGRTRSSSSSSDGSPEAKGGKAGSGRRGEDHPAVMRLKRYIRACGAHRNYKKLLGSCCSHKERLSILRAELEALGMKGTPSLGKCRALKEQREEAAEVASLDVANIISGSGRPRRRTAWNPLGEAAPPGELYRRTLDSDEERPRPAPPDWSHMRGIISSDGESN |
| 分子量 | 88.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HIRIP3蛋白的虚构参考文献示例，格式包括文献名称、作者及摘要概括：
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1. **"Expression and Functional Analysis of Recombinant Human HIRIP3 in DNA Repair Pathways"**
*Smith, J. et al. (2018).*
摘要：本研究成功在大肠杆菌中表达了带有His标签的重组人HIRIP3蛋白，并验证其通过与PCNA和MSH6的相互作用参与DNA错配修复机制。体外实验表明，HIRIP3缺失会导致修复效率下降，提示其调控功能。
2. **"HIRIP3 as a Transcriptional Co-regulator: Insights from Recombinant Protein Studies"**
*Zhang, L. et al. (2020).*
摘要：通过哺乳动物细胞系统表达重组人HIRIP3.结合ChIP-seq分析发现，HIRIP3与RNA聚合酶II及染色质重塑复合物互作，可能通过调控组蛋白修饰影响靶基因(如p53通路基因)的转录活性。
3. **"Structural Characterization of HIRIP3 and Its Role in Hematopoietic Differentiation"**
*Lee, S. et al. (2021).*
摘要：利用杆状病毒系统表达重组HIRIP3.并通过X射线晶体学解析其C端结构域的三维结构。功能实验表明，HIRIP3通过结合转录因子GATA1调控红细胞分化，为血液疾病研究提供新靶点。
4. **"Recombinant HIRIP3 Binds to Mitochondrial Proteins and Modulates Cellular Metabolism"**
*Brown, K. et al. (2022).*
摘要：通过亲和纯化获得高纯度重组HIRIP3蛋白，结合质谱分析发现其与线粒体复合物I组分相互作用。敲低HIRIP3导致细胞ATP生成减少，提示其可能通过能量代谢调控影响癌细胞增殖。
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注：以上参考文献及内容均为虚构，仅作示例用途。实际研究中请核实真实文献。
Human HIRA-interacting protein 3 (HIRIP3) is a nuclear protein that interacts with HIRA, a histone chaperone involved in chromatin remodeling and histone variant deposition. Initially identified through yeast two-hybrid screens, HIRIP3 participates in regulating DNA repair, transcription, and chromatin dynamics. It contains conserved domains, including a CCCH zinc-finger motif, suggesting roles in nucleic acid binding or protein interactions. Studies link HIRIP3 to the HIRA complex, which deposits histone H3.3 into chromatin independently of DNA replication, influencing gene expression and genome stability.
Recombinant human HIRIP3 protein is engineered for in vitro studies to elucidate its molecular functions. Produced via bacterial or mammalian expression systems with affinity tags (e.g., His-tag), it enables biochemical assays, structural analysis, and interaction mapping with partners like HIRA or histones. Research on recombinant HIRIP3 helps dissect its contributions to chromatin remodeling pathways, DNA damage response, and epigenetic regulation. Dysregulation of HIRA complex components, including HIRIP3. is implicated in developmental disorders and cancers, highlighting its biomedical relevance. Functional studies using recombinant protein aid in exploring its potential as a therapeutic target or diagnostic marker in diseases linked to chromatin dysregulation.
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