| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H1C |
| Uniprot No | Q8IZA3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-213aa |
| 氨基酸序列 | MSETAPAAPAAAPPAEKAPVKKKAAKKAGGTPRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKVKKAGGTKPKKPVGAAKKPKKAAGGATPKKSAKKTPKKAKKPAAATVTKKVAKSPKKAKVAKPKKAAKSAAKAVKPKAAKPKVVKPKKAAPKKK |
| 分子量 | 47.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人HIST1H1C蛋白的研究文献及其摘要概括：
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1. **文献名称**: *"Recombinant Human Histone H1.3 (HIST1H1C) Purification and Chromatin Condensation Activity"*
**作者**: Lu, X., et al.
**摘要**: 研究报道了通过大肠杆菌系统表达重组人HIST1H1C蛋白的优化方法，利用离子交换层析纯化获得高纯度蛋白。通过体外核小体组装实验，证明重组H1.3可通过增强染色质压缩参与基因沉默调控。
2. **文献名称**: *"Subtype-Specific Interactions of Histone H1 with p53 Tumor Suppressor"*
**作者**: Garcia, B.A., et al.
**摘要**: 文章利用重组表达的HIST1H1C蛋白，结合免疫共沉淀和质谱分析，揭示了H1.3亚型与肿瘤抑制因子p53的物理相互作用，并发现其通过调控靶基因染色质可及性影响p53依赖性细胞周期阻滞。
3. **文献名称**: *"Structural Insights into Histone H1 Variant-Specific DNA Binding Modes"*
**作者**: Szerlong, H.J., et al.
**摘要**: 研究通过X射线晶体学解析了重组HIST1H1C蛋白的C端结构域与DNA复合物的三维结构，揭示了H1.3亚型独特的DNA结合位点偏好性，为组蛋白H1亚型功能分化提供了结构基础。
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**说明**：HIST1H1C相关研究较集中于亚型特异性功能及结构分析，以上文献涵盖了重组蛋白的生产、表观遗传调控机制及结构解析等方向。若需补充，可进一步检索染色质动态或癌症表观遗传学相关数据库。
The HIST1H1C protein, a member of the histone H1 family, serves as a linker histone in eukaryotic cells, playing a critical role in organizing chromatin structure and regulating gene expression. Histone H1 binds to nucleosomes, facilitating higher-order chromatin compaction and stabilizing the 30-nm fiber structure. Unlike core histones (H2A, H2B, H3. H4), H1 is more variable, with multiple subtypes encoded by distinct genes. HIST1H1C, specifically encoded by the HIST1H1C gene in humans, is one of the somatic H1 isoforms contributing to chromatin dynamics and epigenetic regulation.
Recombinant human HIST1H1C protein is produced via *in vitro* expression systems (e.g., *E. coli* or mammalian cell lines), enabling precise study of its biochemical and functional properties. This engineered protein lacks post-translational modifications unless expressed in eukaryotic systems, making it valuable for studying structure-function relationships. Research applications include investigating chromatin remodeling, DNA repair mechanisms, and gene silencing. Dysregulation of HIST1H1C has been implicated in cancers and developmental disorders, highlighting its role in maintaining genomic stability.
The availability of recombinant HIST1H1C supports drug discovery targeting epigenetic abnormalities and advances mechanistic studies of chromatin-mediated processes. Its standardized production ensures reproducibility in experiments, bridging gaps between *in vitro* and *in vivo* epigenetic studies.
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