| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H2AK |
| Uniprot No | P0C0S8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-130aa |
| 氨基酸序列 | SGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKKTESHHKAKGK |
| 分子量 | 18.0 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HIST1H2AK蛋白的示例参考文献(注：以下内容为假设性文献，供参考使用)：
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1. **文献名称**：*Expression and Structural Characterization of Recombinant Human HIST1H2AK in Escherichia coli*
**作者**：Smith J, et al.
**摘要**：本研究成功在大肠杆菌中克隆并表达了重组人HIST1H2AK蛋白，通过层析技术纯化后，利用圆二色光谱和X射线衍射分析了其二级和三级结构，证实其与天然组蛋白H2A的结构相似性。
2. **文献名称**：*HIST1H2AK Facilitates DNA Repair Complex Assembly in Vitro*
**作者**：Lee S, et al.
**摘要**：通过体外实验探究重组HIST1H2AK蛋白的生物学功能，发现其与DNA损伤位点结合并促进修复复合物(如BRCA1/2)的组装，揭示其在维持基因组稳定性中的潜在作用。
3. **文献名称**：*Epigenetic Regulation by HIST1H2AK in Stem Cell Differentiation*
**作者**：Zhang Y, et al.
**摘要**：该研究利用重组HIST1H2AK蛋白模拟核小体重塑，发现其通过招募组蛋白甲基转移酶(如EZH2)调控多能干细胞分化相关基因的表观遗传修饰。
4. **文献名称**：*HIST1H2AK as a Novel Biomarker for Early Cancer Detection*
**作者**：Patel R, et al.
**摘要**：通过制备重组HIST1H2AK蛋白作为抗原，开发了一种高灵敏度血清检测方法，其在多种癌症患者血清中特异性抗体水平显著升高，显示出临床诊断潜力。
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**注意**：上述文献信息为示例，实际引用需查询真实数据库(如PubMed、Web of Science)获取准确文献。若需进一步协助，建议提供更具体的研究方向或补充关键词。
Recombinant human HIST1H2AK protein is a biologically engineered variant of the histone H2A family, specifically encoded by the HIST1H2AK gene within the HIST1 cluster on chromosome 6. As a core component of nucleosomes, H2A histones play essential roles in chromatin structure and gene regulation by wrapping DNA around histone octamers (two H2A, H2B, H3. and H4 subunits each). HIST1H2AK, a replication-dependent histone, contributes to DNA compaction, epigenetic regulation, and maintenance of genomic stability. Its recombinant form is typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems, enabling high-purity production for research applications. Studies utilize this protein to investigate chromatin dynamics, DNA repair mechanisms, transcriptional regulation, and histone post-translational modifications (e.g., phosphorylation, ubiquitination) linked to diseases like cancer or developmental disorders. Recombinant HIST1H2AK often includes tags (e.g., His, GST) for purification and detection, though tag-free versions are also developed to mimic native protein behavior. Its use in *in vitro* assays, structural studies, and antibody production has advanced understanding of nucleosome remodeling and epigenetic therapeutics. However, the absence of native chromatin context in recombinant systems requires careful experimental validation.
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