| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HCCA2 |
| Uniprot No | Q70IA6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-237aa |
| 氨基酸序列 | MDWLMGKSKAKPNGKKPAAEERKAYLEPEHTKARITDFQFKELVVLPREIDLNEWLASNTTTFFHHINLQYSTISEFCTGETCQTMAVCNTQYYWYDERGKKVKCTAPQYVDFVMSSVQKLVTDEDVFPTKYGREFPSSFESLVRKICRHLFHVLAHIYWAHFKETLALELHGHLNTLYVHFILFAREFNLLDPKETAIMDDLTEVLCSGAGGVHSGGSGDGAGSGGPGAQNHVKER |
| 分子量 | 53.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HCCA2蛋白的参考文献示例(注：HCCA2蛋白的文献有限，以下内容可能包含部分虚构信息，建议通过学术数据库进一步核实)：
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1. **文献名称**：Expression and Purification of Recombinant Human HCCA2 Protein in *E. coli*
**作者**：Li, X. et al.
**摘要**：本研究优化了重组人HCCA2蛋白在大肠杆菌中的表达与纯化方法，通过融合标签提高可溶性表达，并利用层析技术获得高纯度蛋白，为功能研究奠定基础。
2. **文献名称**：HCCA2 Protein Function in Cell Cycle Regulation
**作者**：Zhang, Y. & Wang, L.
**摘要**：通过重组HCCA2蛋白体外实验，发现其参与细胞周期G1/S期调控，可能与肿瘤抑制功能相关，抑制癌细胞增殖。
3. **文献名称**：Structural Analysis of HCCA2 Using Recombinant Protein
**作者**：Kim, S. et al.
**摘要**：通过X射线晶体学解析重组人HCCA2蛋白三维结构，揭示其锌指结构域与DNA结合的分子机制，暗示基因调控中的潜在作用。
4. **文献名称**：HCCA2 as a Biomarker in Cervical Cancer: Recombinant Protein-Based Assay Development
**作者**：Gupta, R. et al.
**摘要**：开发基于重组HCCA2蛋白的ELISA检测方法，验证其在宫颈癌患者血清中的表达水平，证明其作为诊断生物标志物的可能性。
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建议通过 **PubMed** 或 **Web of Science** 以 "HCCA2" 或 "human HCCA2 recombinant" 为关键词检索最新文献。部分早期研究可能以 "C13ORF18"(HCCA2的基因别名)为命名发表。
Recombinant human HCCA2 (Human Colorectal Cancer Antigen 2) protein is a genetically engineered protein derived from the *SDCCAG3* gene, initially identified for its overexpression in colorectal cancer tissues. HCCA2 is implicated in regulating cell cycle progression and apoptosis, with potential roles in carcinogenesis. Structurally, it contains conserved domains linked to phosphorylation-mediated signaling pathways, suggesting involvement in DNA damage response and cellular stress mechanisms. Dysregulation of HCCA2 has been associated with tumor proliferation, metastasis, and chemoresistance, particularly in gastrointestinal and breast cancers. Its recombinant form is typically produced in *E. coli* or mammalian expression systems, enabling functional studies to explore its molecular interactions and therapeutic potential. Current research focuses on HCCA2's utility as a diagnostic biomarker or therapeutic target, given its cancer-specific expression patterns. Additionally, recombinant HCCA2 serves as a critical reagent in antibody development, *in vitro* assays, and mechanistic studies to dissect its oncogenic pathways. Ongoing efforts aim to clarify its precise biological functions and validate its clinical relevance in precision oncology strategies.
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