| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HAUS5 |
| Uniprot No | O94927 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-633aa |
| 氨基酸序列 | MELAQEARELGCWAVEEMGVPVAARAPESTLRRLCLGQGADIWAYILQHVHSQRTVKKIRGNLLWYGHQDSPQVRRKLELEAAVTRLRAEIQELDQSLELMERDTEAQDTAMEQARQHTQDTQRRALLLRAQAGAMRRQQHTLRDPMQRLQNQLRRLQDMERKAKVDVTFGSLTSAALGLEPVVLRDVRTACTLRAQFLQNLLLPQAKRGSLPTPHDDHFGTSYQQWLSSVETLLTNHPPGHVLAALEHLAAEREAEIRSLCSGDGLGDTEISRPQAPDQSDSSQTLPSMVHLIQEGWRTVGVLVSQRSTLLKERQVLTQRLQGLVEEVERRVLGSSERQVLILGLRRCCLWTELKALHDQSQELQDAAGHRQLLLRELQAKQQRILHWRQLVEETQEQVRLLIKGNSASKTRLCRSPGEVLALVQRKVVPTFEAVAPQSRELLRCLEEEVRHLPHILLGTLLRHRPGELKPLPTVLPSIHQLHPASPRGSSFIALSHKLGLPPGKASELLLPAAASLRQDLLLLQDQRSLWCWDLLHMKTSLPPGLPTQELLQIQASQEKQQKENLGQALKRLEKLLKQALERIPELQGIVGDWWEQPGQAALSEELCQGLSLPQWRLRWVQAQGALQKLCS |
| 分子量 | 69.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HAUS5蛋白的3条参考文献摘要整理(虚构示例，仅供格式参考)：
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1. **文献名称**：The human HAUS complex is required for mitotic spindle assembly and chromosome segregation
**作者**：Lawo S, et al.
**摘要**：本文首次鉴定了人类HAUS复合体的8个核心亚基(包括HAUS5)，并证明其与Augmin复合体协作促进微管形成。研究发现，HAUS5的缺失会导致纺锤体微管密度降低和染色体排列异常，表明其在维持基因组稳定性中起关键作用。
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2. **文献名称**：Functional characterization of HAUS5 in cancer cell proliferation
**作者**：Matsumoto Y, et al.
**摘要**：通过RNA干扰技术敲低HAUS5后，发现乳腺癌细胞有丝分裂阻滞增加并伴随凋亡。研究证实HAUS5通过调控纺锤体完整性影响细胞周期进程，提示其可能作为癌症治疗的潜在靶点。
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3. **文献名称**：Recombinant HAUS5 protein purification and microtubule binding assay
**作者**：Uehara R, et al.
**摘要**：本研究成功在大肠杆菌中表达重组人HAUS5蛋白，并利用体外微管共沉淀实验证明其直接结合α-微管蛋白。该纯化系统为后续HAUS复合体的功能解析提供了可靠工具。
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注：以上文献信息为基于领域常识的模拟数据，实际引用请以真实文献为准。建议在PubMed或Web of Science中以“HAUS5”和“mitotic spindle”为关键词检索最新研究。
**Background of Recombinant Human HAUS5 Protein**
The HAUS5 protein, a critical subunit of the HAUS (homologous to augmin subunits) complex, plays a vital role in mitotic spindle assembly and microtubule organization. This complex facilitates γ-tubulin recruitment to spindle microtubules, ensuring proper chromosome segregation during cell division. HAUS5. also termed DGT6. interacts with other augmin subunits to stabilize microtubule arrays, enabling spindle formation in a centrosome-independent manner. Dysregulation of HAUS5 is linked to mitotic errors, genomic instability, and diseases such as cancer.
Recombinant HAUS5 protein, produced via expression systems like *E. coli* or mammalian cells, retains functional properties for structural and mechanistic studies. Its production enables exploration of HAUS5's interactions within the augmin complex, microtubule dynamics, and regulatory phosphorylation events. Researchers utilize this tool to dissect HAUS5’s roles in cell cycle progression, cancer biology, and developmental disorders.
Studies highlight HAUS5's involvement in human pathologies; for instance, overexpression correlates with poor prognosis in certain cancers. Recombinant HAUS5 also aids in drug screening for therapies targeting mitotic machinery. Additionally, structural analyses reveal conserved TPR (tetratricopeptide repeat) domains critical for protein interactions. Overall, recombinant HAUS5 serves as a pivotal reagent for advancing mitotic biology and therapeutic innovation.
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