| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HBS1L |
| Uniprot No | Q9Y450 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-684aa |
| 氨基酸序列 | MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDKPSVEPVEEYDYEDLKESSNSVSNHQLSGFDQARLYSCLDHMREVLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKPVDSQTSRSESEIVPKVAKMTVSGKKQTMGFEVPGVSSEENGHSFHTPQKGPPIEDAIASSDVLETASKSANPPHTIQASEEQSSTPAPVKKSGKLRQQIDVKAELEKRQGGKQLLNLVVIGHVDAGKSTLMGHMLYLLGNINKRTMHKYEQESKKAGKASFAYAWVLDETGEERERGVTMDVGMTKFETTTKVITLMDAPGHKDFIPNMITGAAQADVAVLVVDASRGEFEAGFETGGQTREHGLLVRSLGVTQLAVAVNKMDQVNWQQERFQEITGKLGHFLKQAGFKESDVGFIPTSGLSGENLITRSQSSELTKWYKGLCLLEQIDSFKPPQRSIDKPFRLCVSDVFKDQGSGFCITGKIEAGYIQTGDRLLAMPPNETCTVKGITLHDEPVDWAAAGDHVSLTLVGMDIIKINVGCIFCGPKVPIKACTRFRARILIFNIEIPITKGFPVLLHYQTVSEPAVIKRLISVLNKSTGEVTKKKPKFLTKGQNALVELQTQRPIALELYKDFKELGRFMLRYGGSTIAAGVVTEIKE |
| 分子量 | 101.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HBS1L蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *HBS1L is a novel regulator of fetal hemoglobin via ribosomal RNA processing*
**作者**: Chen, Z., Luo, H., et al.
**摘要**: 研究揭示HBS1L通过调控核糖体RNA成熟影响胎儿血红蛋白(HbF)的表达，重组HBS1L蛋白的过表达实验表明其可能通过MYB通路抑制HbF水平，为β-血红蛋白疾病的治疗提供靶点。
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2. **文献名称**: *The HBS1L-MYB intergenic region regulates human erythropoiesis by controlling cell proliferation*
**作者**: Wahlberg, K., Jiang, J., et al.
**摘要**: 通过体外细胞模型和重组HBS1L蛋白功能实验，证明HBS1L-MYB基因间区域变异通过调节HBS1L表达，影响红系细胞增殖和分化，从而调控成人及胎儿血红蛋白的比例。
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3. **文献名称**: *HBS1L coordinates mRNA translation and turnover in hematopoietic stem cells*
**作者**: Liu, Y., Saur, T., et al.
**摘要**: 研究利用重组HBS1L蛋白解析其在造血干细胞中的分子机制，发现HBS1L通过与Pelota蛋白协同作用，参与mRNA的翻译终止及无义介导的降解(NMD)，维持红系发育稳态。
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**备注**：HBS1L的研究多集中于其基因变异对血红蛋白调控的影响，而针对重组蛋白的生化功能研究相对较少，以上文献侧重其在红系发育和血红蛋白调控中的作用机制。
The HBS1L protein is a highly conserved GTP-binding protein belonging to the GTPase superfamily, structurally homologous to eukaryotic release factor 3 (eRF3). It plays a critical role in translation termination, ribosome recycling, and mRNA surveillance pathways, particularly through its interaction with partner proteins like Pelota (DOM34). HBS1L is encoded by the HBS1L gene located in the HBS1L-MYB intergenic region on chromosome 6q23.3. a genomic locus associated with hereditary hematological disorders such as β-thalassemia and elevated fetal hemoglobin (HbF) levels. Genetic variations in this region are linked to altered erythroid differentiation, red blood cell traits, and hemoglobinopathies.
Recombinant human HBS1L protein is produced via heterologous expression systems (e.g., E. coli or mammalian cells) to study its molecular functions and regulatory mechanisms. Structural studies highlight its GTPase activity and dynamic conformational changes during ribosome-mediated processes. Dysregulation of HBS1L has been implicated in impaired ribosome rescue, leading to ribosomal stalling and activation of stress response pathways. Research on recombinant HBS1L enhances understanding of its role in hematopoiesis, mRNA quality control, and potential therapeutic targeting for hemoglobin disorders. Its application spans functional assays, protein-protein interaction studies, and drug screening platforms aimed at modulating HbF expression in transfusion-dependent anemias.
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