| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HAN11 |
| Uniprot No | P61962 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-342aa |
| 氨基酸序列 | MSLHGKRKEIYKYEAPWTVYAMNWSVRPDKRFRLALGSFVEEYNNKVQLVGLDEESSEFICRNTFDHPYPTTKLMWIPDTKGVYPDLLATSGDYLRVWRVGETETRLECLLNNNKNSDFCAPLTSFDWNEVDPYLLGTSSIDTTCTIWGLETGQVLGRVNLVSGHVKTQLIAHDKEVYDIAFSRAGGGRDMFASVGADGSVRMFDLRHLEHSTIIYEDPQHHPLLRLCWNKQDPNYLATMAMDGMEVVILDVRVPCTPVARLNNHRACVNGIAWAPHSSCHICTAADDHQALIWDIQQMPRAIEDPILAYTAEGEINNVQWASTQPDWIAICYNNCLEILRV |
| 分子量 | 54.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HAN11蛋白的假设性参考文献示例，供参考：
1. **《重组人HAN11蛋白在大肠杆菌中的表达与纯化》**
- 作者：张伟，李明，王芳
- 摘要：本研究成功构建了HAN11基因的原核表达载体，利用大肠杆菌系统进行诱导表达，并通过镍柱亲和层析纯化获得高纯度的重组蛋白。Western blot验证其免疫活性，为后续功能研究奠定基础。
2. **《HAN11蛋白在DNA损伤修复中的功能机制研究》**
- 作者：Johnson A.R., Smith T.K.
- 摘要：通过体外实验发现，重组人HAN11蛋白与Rad51相互作用，增强同源重组修复效率。敲低HAN11的细胞对电离辐射敏感，提示其在基因组稳定性中的关键作用。
3. **《HAN11蛋白的结构解析及其与癌症的关联》**
- 作者：Li X., Chen Z., Liu Y.
- 摘要：采用X射线晶体学解析HAN11蛋白的三维结构，揭示其特有的锌指结构域。临床样本分析显示，HAN11在乳腺癌中表达异常，可能作为潜在治疗靶点。
4. **《哺乳动物细胞中重组HAN11蛋白的表达及功能验证》**
- 作者：Patel S., Gupta R.
- 摘要：在HEK293细胞中瞬转表达带有Flag标签的HAN11蛋白，验证其定位于细胞核。功能实验表明，HAN11过表达抑制细胞周期进程，暗示其参与增殖调控。
**注意**：上述文献为假设性示例，实际研究中请通过学术数据库(如PubMed、Google Scholar)以“HAN11”、“重组表达”、“功能研究”等关键词检索准确信息。
Recombinant human HAN11 protein is a genetically engineered protein derived from the *HAN11* gene (also called *HSPC068* or *TSGA14*), which encodes a conserved yet poorly characterized scaffold protein. HAN11 plays regulatory roles in diverse cellular processes, including cell cycle progression, apoptosis, and stress signaling. Studies suggest it serves as an adaptor molecule for the tumor suppressor RASSF1A (Ras association domain-containing protein 1A), forming a complex that modulates the Hippo signaling pathway by facilitating interactions between RASSF1A and YAP1 (Yes-associated protein 1). This interaction influences cell proliferation and organ size regulation. HAN11 is also implicated in DNA damage response and mitotic regulation, possibly through its phosphorylation-dependent interactions with kinases. Its dysregulation has been linked to cancer progression, neurodevelopmental disorders, and cardiovascular diseases. Recombinant HAN11. typically produced in *E. coli* or mammalian expression systems, retains functional domains necessary for protein-protein interactions and post-translational modifications. It is widely used in biochemical assays, structural studies, and mechanistic research to unravel its roles in signaling networks, offering potential therapeutic insights. Further characterization of HAN11 may clarify its tissue-specific functions and disease associations.
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