| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HARSL |
| Uniprot No | P49590 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 34-506aa |
| 氨基酸序列 | QSQVAEAVLTSQLKAHQEKPNFIIKTPKGTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES |
| 分子量 | 69.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HARSL蛋白的几篇假设性参考文献示例(注：HARSL相关研究较少，实际文献可能需通过专业数据库核实)：
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1. **文献名称**: *Expression and Purification of Recombinant Human HARSL Protein for Functional Analysis*
**作者**: Zhang, Y. et al.
**摘要**: 本研究报道了人源HARSL蛋白在大肠杆菌中的重组表达与纯化，并证明其具有精氨酰-tRNA合成酶活性，为研究其在线粒体功能中的作用提供了工具。
2. **文献名称**: *Structural Insights into Human HARSL by X-ray Crystallography*
**作者**: Lee, S. & Kim, T.
**摘要**: 通过重组表达HARSL蛋白并进行晶体结构解析，揭示了其与线粒体tRNA的特异性结合位点，提示其突变可能与罕见遗传病相关。
3. **文献名称**: *HARSL Knockdown in Zebrafish Models Neurological Defects Rescued by Recombinant Protein*
**作者**: Garcia, R. et al.
**摘要**: 在斑马鱼模型中，重组人HARSL蛋白成功挽救了由基因敲除引起的神经系统异常，表明其可能参与神经发育调控。
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**注意事项**：
- HARSL(如指线粒体亮氨酰-tRNA合成酶，别名LARS2)的研究多集中于基因突变与疾病(如佩梅病)的关联，重组蛋白的直接研究较少。
- 建议通过PubMed/Google Scholar搜索 **“recombinant LARS2”** 或 **“HARSL protein expression”** 获取真实文献。
- 确认HARSL是否为“HARS”(组氨酰-tRNA合成酶)的拼写误差，后者重组表达研究较多。
Human histidyl-tRNA synthetase-like (HARSL) protein, encoded by the *HARS2* gene, is a member of the aminoacyl-tRNA synthetase (ARS) family. These enzymes are essential for protein synthesis, catalyzing the attachment of specific amino acids to their cognate tRNAs. HARSL shares structural homology with histidyl-tRNA synthetase (HARS1) but exhibits distinct functional roles, possibly linked to its non-canonical subcellular localization or moonlighting functions. Unlike most ARSs, HARSL lacks catalytic activity for aminoacylation, suggesting evolutionary divergence toward regulatory or signaling roles.
Research implicates HARSL in mitochondrial function, as *HARS2* mutations are associated with Perrault syndrome (a rare disorder characterized by hearing loss and ovarian dysfunction) and neurodegenerative conditions. Its mitochondrial isoform may contribute to tRNA maturation or translation fidelity within organelles. Recombinant human HARSL protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) for biochemical studies, structural analysis, and therapeutic exploration. Investigations focus on elucidating its interactome, post-translational modifications, and pathological mechanisms in ARS-related diseases. Recombinant forms are also tools for developing targeted therapies, including enzyme replacement or gene-editing strategies. Studies continue to unravel HARSL’s roles beyond translation, potentially linking it to cellular stress responses or metabolic regulation.
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