| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GOLGA2 |
| Uniprot No | Q08379 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-345aa |
| 氨基酸序列 | MQNDRTTISRALSQNRELKEQLAELQSGFVKLTNENMEITSALQSEQHVKRELGKKLGELQEKLSELKETVELKSQEAQSLQQQRDQYLGHLQQYVAAYQQLTSEKEVLHNQLLLQTQLVDQLQQQEAQGKAVAEMARQELQETQERLEAATQQNQQLRAQLSLMAHPGEGDGLDREEEEDEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQSVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERPGRVLPVTTPLHSRSCSCFVRCRTPGSAQAWAATPAFLFFTGLTRMMR |
| 分子量 | 63.58 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人GOLGA2蛋白的模拟参考文献(基于研究背景虚拟描述，实际文献需自行检索补充)：
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1. **文献名称**: *"Expression and Purification of Recombinant Human GOLGA2 in a Mammalian System"*
**作者**: Zhang Y. et al.
**摘要**: 研究报道了在HEK293细胞中高效表达重组人GOLGA2蛋白的方法，通过融合His标签进行亲和层析纯化，并验证了其在高尔基体相关膜蛋白运输中的功能性。
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2. **文献名称**: *"GOLGA2 Interaction with Rab GTPases in Vesicular Trafficking"*
**作者**: Tanaka K. et al.
**摘要**: 利用重组GOLGA2蛋白进行体外结合实验，证明其与Rab1和Rab6的相互作用，揭示其在调控高尔基体-内质网囊泡运输中的分子机制。
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3. **文献名称**: *"Role of Recombinant GOLGA2 in Cancer Cell Migration"*
**作者**: Lee S. et al.
**摘要**: 研究发现重组GOLGA2蛋白在癌细胞中过表达可促进迁移，通过激活Wnt/β-catenin信号通路，提示其作为潜在肿瘤治疗靶点。
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4. **文献名称**: *"Structural Analysis of GOLGA2’s Coiled-Coil Domains by Cryo-EM"*
**作者**: Müller R. et al.
**摘要**: 通过冷冻电镜解析重组GOLGA2蛋白的卷曲螺旋结构域，阐明了其在维持高尔基体堆叠结构中的关键作用，并提出了动态组装模型。
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如需真实文献，建议在 **PubMed** 或 **Google Scholar** 中检索关键词：*recombinant human GOLGA2 protein* 或 *GOLGA2 expression/purification/function*。
Recombinant human GOLGA2 protein, also known as golgin-245 or GM130. is a Golgi-associated protein involved in maintaining Golgi structure and regulating vesicular trafficking. It is encoded by the GOLGA2 gene and plays a critical role in organizing the cisternal stacking of the Golgi apparatus, facilitating protein transport between the endoplasmic reticulum and Golgi. GOLGA2 contains multiple coiled-coil domains and a GRIP domain, enabling interactions with other golgins and Rab GTPases. Studies suggest its involvement in cell cycle progression, apoptosis, and response to cellular stress. Dysregulation of GOLGA2 has been linked to cancers, neurodegenerative disorders, and infectious diseases. Recombinant GOLGA2 is typically produced in expression systems like E. coli or mammalian cells, often fused with tags (e.g., His-tag) for purification. It serves as a vital tool for studying Golgi dynamics, protein-protein interactions, and disease mechanisms. Its structure-function relationships are investigated using techniques such as co-immunoprecipitation and microscopy. Researchers also utilize recombinant GOLGA2 to generate antibodies or probe its role in viral replication, where some pathogens exploit Golgi components for intracellular survival.
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