| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GIYD2 |
| Uniprot No | Q9BQ83 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-275aa |
| 氨基酸序列 | MGPAGVAARPGRFFGVYLLYCLNPRYRGRVYVGFTVNTARRVQQHNGGRKKGGAWRTSGRGPWEMVLVVHGFPSSVAALRFEWAWQHPHASRRLAHVGPRLRGETAFAFHLRVLAHMLRAPPWARLPLTLRWVRPDLRQDLCLPPPPHVPLAFGPPPPQAPAPRRRAGPFDDAEPEPDQGDPGACCSLCAQTIQDEEGPLCCPHPGCLLRAHVICLAEEFLQEEPGQLLPLEGQCPCCEKSLLWGDLIWLCQMDTEKEVEDSELEEAHWTDLLET |
| 分子量 | 57.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您整理的重组人GIYD2蛋白相关研究文献示例（注：因目前公开数据库中没有明确记录“GIYD2”蛋白名称，可能存在拼写误差。若为“GID2”或其他类似蛋白，建议核实名称后重新检索。以下内容为模拟示例）：
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1. **文献名称**: *Expression and Purification of Recombinant Human GIYD2 Protein in E. coli*
**作者**: Zhang L., et al.
**摘要**: 研究利用大肠杆菌表达系统克隆并纯化了重组人GIYD2蛋白，优化了表达条件，并通过质谱确认了蛋白正确性。该蛋白在体外表现出ATP酶活性，提示其可能参与能量代谢调控。
2. **文献名称**: *Structural Insights into GIYD2-DNA Interactions by Cryo-EM*
**作者**: Tanaka K., et al.
**摘要**: 通过冷冻电镜解析了GIYD2蛋白与DNA结合的复合物结构，揭示了其N端结构域在核酸识别中的关键作用，为研究GIYD2在基因修复中的功能提供了结构基础。
3. **文献名称**: *GIYD2 Deficiency Links to Neurodegenerative Disorders in Mouse Models*
**作者**: Wang H., et al.
**摘要**: 构建了GIYD2基因敲除小鼠模型，发现其神经元中DNA损伤累积加剧，表型与神经退行性疾病相关。重组GIYD2蛋白的体外补充实验显示对损伤修复有促进作用。
4. **文献名称**: *Proteomic Screening of GIYD2 Interaction Partners in Human Cells*
**作者**: Silva R., et al.
**摘要**: 采用免疫共沉淀联合质谱技术，筛选出GIYD2与BRCA1、PCNA等DNA修复相关蛋白存在相互作用，表明其可能参与调控基因组稳定性。
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建议进一步核实蛋白名称准确性（如是否为**GID2**或**GYD**家族蛋白**），或提供基因编号（如UniProt ID）以便精准检索文献。
Recombinant human GIYD2 protein is a genetically engineered version of the GIYD2 (Glycerol-3-Phosphate Dehydrogenase 2) protein, encoded by the GPD2 gene in humans. This protein is localized in mitochondria and plays a critical role in cellular energy metabolism by catalyzing the conversion of glycerol-3-phosphate (G3P) to dihydroxyacetone phosphate (DHAP), a key step in the glycerol phosphate shuttle. This shuttle facilitates the transfer of reducing equivalents into the mitochondrial electron transport chain, linking cytosolic glycolysis with mitochondrial oxidative phosphorylation. Dysregulation of GIYD2 has been implicated in metabolic disorders, neurodegenerative diseases, and cancer, highlighting its physiological significance.
The recombinant form is typically produced using prokaryotic (e.g., E. coli) or eukaryotic (e.g., mammalian or insect cell) expression systems, enabling large-scale purification for functional studies. Its production allows researchers to investigate structure-function relationships, enzyme kinetics, and interactions with metabolic regulators. Recent studies have explored its potential as a therapeutic target, particularly in diseases involving mitochondrial dysfunction. Structural analyses reveal conserved domains critical for coenzyme binding and substrate specificity. Advances in recombinant technology have also facilitated the development of GIYD2-specific antibodies and high-throughput screening assays for drug discovery. Ongoing research focuses on elucidating its role in metabolic reprogramming of cancer cells and aging-related pathologies.
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