| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GIYD1 |
| Uniprot No | Q9BQ83 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-275aa |
| 氨基酸序列 | MGPAGVAARP GRFFGVYLLY CLNPRYRGRV YVGFTVNTAR RVQQHNGGRK KGGAWRTSGR GPWEMVLVVH GFPSSVAALR FEWAWQHPHA SRRLAHVGPR LRGETAFAFH LRVLAHMLRA PPWARLPLTL RWVRPDLRQD LCLPPPPHVP LAFGPPPPQA PAPRRRAGPF DDAEPEPDQG DPGACCSLCA QTIQDEEGPL CCPHPGCLLR AHVICLAEEF LQEEPGQLLP LEGQCPCCEK SLLWGDLIWL CQMDTEKEVE DSELEEAHWT DLLET |
| 分子量 | 30.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GIYD1蛋白的3篇示例参考文献，由于该蛋白名称可能冷门或存在拼写问题，文献内容为模拟概括，仅供参考格式：
1. **文献名称**：Expression and purification of recombinant human GIYD1 protein in E. coli
**作者**：Zhang L, et al.
**摘要**：本研究利用大肠杆菌系统表达了带有His标签的重组人GIYD1蛋白，优化了诱导条件和纯化步骤，最终通过亲和层析获得高纯度蛋白，并验证了其体外酶活性。
2. **文献名称**：Functional characterization of GIYD1 in nucleotide metabolism
**作者**：Wang Y, et al.
**摘要**：通过重组GIYD1蛋白的体外实验，发现其具有水解特定核苷酸类似物的功能，提示其可能参与DNA修复通路，为癌症靶向治疗提供潜在靶点。
3. **文献名称**：Structural insights into human GIYD1 by X-ray crystallography
**作者**：Smith J, et al.
**摘要**：解析了重组GIYD1蛋白的晶体结构，揭示了其底物结合口袋的三维构象，并探讨了关键氨基酸残基在催化反应中的作用机制。
**注意**：以上文献为示例，实际研究中建议通过 **PubMed/Google Scholar** 检索关键词（如 "recombinant human GIYD1"）或核实蛋白名称（如是否混淆GID、GIGYF1等同源蛋白）。若为冷门靶点，可扩展至相关家族蛋白研究或方法学文献（如重组蛋白表达通用策略）。
Recombinant human GIYD1 protein is a genetically engineered form of the GIY-YIG domain-containing protein 1 encoded by the *GIYD1* gene in humans. GIYD1 belongs to the GIY-YIG nuclease superfamily, characterized by conserved catalytic motifs involved in DNA cleavage and repair processes. Though its exact physiological role remains under investigation, studies suggest its potential involvement in maintaining genome stability, possibly through DNA repair pathways or resolving replication stress. The Y-linked *GIYD1* gene, located on the Y chromosome, has drawn attention in research related to male-specific biological processes, including spermatogenesis, and its dysfunction may link to fertility disorders or cancer susceptibility.
Recombinant GIYD1 is typically produced in prokaryotic (e.g., *E. coli*) or eukaryotic expression systems to ensure proper folding and post-translational modifications. It serves as a vital tool for in vitro studies to elucidate its biochemical activities, such as DNA-binding specificity, endonuclease function, and interactions with other repair proteins. Its recombinant form also aids in structural biology (e.g., crystallography) to map functional domains and catalytic mechanisms. Emerging research explores its diagnostic or therapeutic potential, particularly in contexts of genomic instability diseases. However, further functional characterization is required to fully define its role in cellular pathways and human health.
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