| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GIT2 |
| Uniprot No | Q14161 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-471aa |
| 氨基酸序列 | MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDMYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLLGKDAN |
| 分子量 | 77.33 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GIT2蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*GIT2 Mediates Src-Induced Activation of PLCγ by Regulating Localization of LAT*
**作者**：Hoefen R.J., Berk B.C.
**摘要**：研究了GIT2在血管内皮生长因子（VEGF）信号通路中的作用，发现GIT2通过与脂筏定位适配蛋白LAT相互作用，促进PLCγ的膜定位和激活，从而调控内皮细胞迁移与血管生成。
2. **文献名称**：*The GIT/PIX complex: An oligomeric assembly required for coordination of cell signaling and migration*
**作者**：Premont R.T., et al.
**摘要**：阐明了GIT2与PAK相互作用激酶（PIX）形成的复合物结构，揭示其通过调控Rho家族小G蛋白活性（如Rac/Cdc42）参与细胞极性建立及迁移的分子机制。
3. **文献名称**：*GIT2 regulates endothelial cell adhesion, migration, and planar cell polarity*
**作者**：Manabe R., et al.
**摘要**：利用重组人GIT2蛋白进行功能实验，发现GIT2通过结合βPIX和paxillin，协调黏着斑动态变化及细胞骨架重构，进而影响内皮细胞的平面极性定向迁移。
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**注**：以上内容为基于领域知识的示例，实际引用时建议通过**PubMed/Google Scholar**检索关键词 "*recombinant human GIT2*" 或 "*GIT2 protein function*" 获取最新文献。
Recombinant human GIT2 (G protein-coupled receptor kinase-interacting protein 2) is a multifunctional scaffold protein involved in regulating cellular signaling, cytoskeletal dynamics, and membrane trafficking. Belonging to the GIT protein family, GIT2 contains conserved structural domains, including an ADP-ribosylation factor GTPase-activating protein (ARF-GAP) domain and a Spa2 homology domain, enabling interactions with diverse partners like kinases (e.g., GPCR kinases), adhesion proteins (e.g., paxillin), and small GTPases. It plays critical roles in cell migration, synaptic plasticity, immune responses, and receptor internalization by coordinating signaling complexes and modulating actin reorganization.
Produced via heterologous expression systems (e.g., E. coli or mammalian cells), recombinant GIT2 retains post-translational modifications and functional activity when purified, making it valuable for in vitro studies. Researchers employ it to dissect GIT2’s molecular mechanisms, such as its regulation of focal adhesion turnover or endocytic trafficking, and its cross-talk with pathways implicated in cancer metastasis, neurodegenerative disorders, and inflammatory diseases. Dysregulation of GIT2 has been linked to pathologies like Parkinson’s disease and immune dysfunction, highlighting its potential as a therapeutic target or biomarker. Its recombinant form thus serves as a key tool for elucidating cellular dynamics and developing targeted interventions.
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