| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GIMAP8 |
| Uniprot No | Q8ND71 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-665aa |
| 氨基酸序列 | MSEQSCQMSELRLLLLGKCRSGKSATGNAILGKHVFKSKFSDQTVIKMCQRESWVLRERKVVVIDTPDLFSSIACAEDKQRNIQHCLELSAPSLHALLLVIAIGHFTREDEETAKGIQQVFGAEARRHIIIVFTRKDDLGDDLLQDFIEKNKPLKQLVQDYEGRYCIFNNKTNSKDEQITQVLELLRKVESLVNTNGGPYHVNFKTEGSRFQDCVNEAASQEGDKPQGPRERQLQSTGPEQNPGTSELTVLLVGKRGAGKSAAGNSILGRQAFQTGFSEQSVTQSFLSESRSWRKKKVSIIDAPDISSLKNIDSEVRKHICTGPHAFLLVTPLGFYTKNDEAVLSTIQNNFGEKFFEYMIILLTRKEDLGDQDLDTFLRNSNKALYGLIQKCKNRYSAFNYRATGEEEQRQADELLEKIESMVHQNGNKHCVFREKETLNIVLVGRSGTGKSATGNSILGSLVFTSRLRAQPVTKTSQSGRRTWDGQEVVVVDTPSFNQMLDVEKDPSRLEEEVKRCLSCCEKGDTFFVLVFQLGRFTEEDKTAVAKLEAIFGADFTKYAIMLFTRKEDLGAGNLEDFMKNSDNKALRRIFKKCGRRVCAFNNKETGQAQETQVKALLTKVNDLRKESGWSGYPHTQENVSKLIKNVQEMSQAEKLLKNLIGILQ |
| 分子量 | 101.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GIMAP8蛋白的3篇文献概要(注：以下内容基于学术研究的常见方向模拟整理，实际文献可能需要通过数据库查询确认)：
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1. **文献名称**: *Structural Insights into the GTPase Domain of Human GIMAP8*
**作者**: Schwefel, D. et al.
**摘要**: 该研究通过X射线晶体学解析了人GIMAP8蛋白的GTP酶结构域三维结构，揭示了其活性位点的关键氨基酸残基，并探讨了重组表达的GIMAP8在GTP水解中的功能机制。
2. **文献名称**: *Expression and Purification of Recombinant Human GIMAP8 in E. coli for Functional Analysis*
**作者**: Müller, R. et al.
**摘要**: 文章优化了在大肠杆菌系统中重组表达和纯化人GIMAP8蛋白的流程，验证了其体外稳定性，并证明纯化后的蛋白可用于后续免疫印迹和酶活实验。
3. **文献名称**: *GIMAP8 Modulates T Cell Survival via Interaction with Bcl-2 Family Proteins*
**作者**: Pandey, A. et al.
**摘要**: 研究发现重组人GIMAP8蛋白通过与Bcl-2家族蛋白相互作用调控T细胞凋亡，实验中使用HEK293细胞表达的重组蛋白揭示了其在维持免疫稳态中的潜在作用。
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如需获取真实文献，建议访问 **PubMed** 或 **Google Scholar** 并检索关键词：
`"recombinant human GIMAP8"` 或 `"GIMAP8 protein expression"`。
GIMAP8 (GTPase, IMAP family member 8) is a member of the GTP-binding immune-associated protein (GIMAP) family, predominantly expressed in immune cells, particularly lymphocytes. This protein plays a regulatory role in intracellular processes linked to immune homeostasis, autophagy, and cell survival. Structurally, it contains a conserved GTP-binding domain critical for its molecular switch function, modulating interactions with downstream effectors. Studies suggest GIMAP8 localizes to autophagosomal membranes, influencing autophagic flux and lysosomal degradation—a mechanism implicated in removing damaged organelles and pathogens. Dysregulation of GIMAP8 has been associated with autoimmune disorders, lymphopenia, and aberrant T-cell responses, highlighting its importance in immune tolerance.
Recombinant human GIMAP8 protein is engineered for functional studies, enabling researchers to investigate its enzymatic activity, protein interactions, and role in immune signaling pathways. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains post-translational modifications essential for biological activity when using eukaryotic hosts. Current research focuses on its potential as a therapeutic target for immune-mediated diseases and cancer, given its involvement in stress-induced apoptosis and immune cell survival. Further exploration of GIMAP8 could unveil mechanisms underlying immune dysregulation and autophagy-related pathologies.
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