| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GIMAP4 |
| Uniprot No | Q9NUV9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-329aa |
| 氨基酸序列 | MAAQYGSMSFNPSTPGASYGPGRQEPRNSQLRIVLVGKTGAGKSATGNSILGRKVFHSGTAAKSITKKCEKRSSSWKETELVVVDTPGIFDTEVPNAETSKEIIRCILLTSPGPHALLLVVPLGRYTEEEHKATEKILKMFGERARSFMILIFTRKDDLGDTNLHDYLREAPEDIQDLMDIFGDRYCALNNKATGAEQEAQRAQLLGLIQRVVRENKEGCYTNRMYQRAEEEIQKQTQAMQELHRVELEREKARIREEYEEKIRKLEDKVEQEKRKKQMEKKLAEQEAHYAVRQQRARTEVESKDGILELIMTALQIASFILLRLFAED |
| 分子量 | 63.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于重组人GIMAP4蛋白的参考文献示例：
1. **标题**：**"Recombinant human GIMAP4 induces T-cell survival through regulation of mitochondrial membrane potential"**
**作者**：Li et al. (2016)
**摘要**：研究报道了在昆虫细胞系统中表达重组人GIMAP4蛋白，并证实其通过稳定线粒体膜电位抑制T细胞凋亡，提示其在免疫调节中的作用。
2. **标题**：**"Functional characterization of recombinant GIMAP4 in autophagy regulation"**
**作者**：Huang et al. (2020)
**摘要**：利用HEK293细胞表达重组GIMAP4蛋白，发现其通过与自噬相关蛋白互作调控自噬体形成，可能影响细胞应激反应。
3. **标题**：**"Crystal structure and GTPase activity of human GIMAP4: Insights into its molecular mechanism"**
**作者**：Zhang et al. (2022)
**摘要**：通过X射线晶体学解析重组人GIMAP4的三维结构，揭示其GTP酶结构域的关键功能位点，并验证其对GTP水解的催化活性。
4. **标题**：**"Recombinant GIMAP4 suppresses NLRP3 inflammasome activation in macrophages"**
**作者**：Wang et al. (2023)
**摘要**：体外实验显示，重组人GIMAP4蛋白通过抑制NLRP3炎症小体组装，减轻炎症因子释放，或为炎症性疾病提供潜在治疗策略。
(注：上述文献为示例，实际引用需根据具体论文调整。建议通过PubMed或Google Scholar以关键词“recombinant GIMAP4 human”检索最新研究。)
GIMAP4 (GTPase, IMAP family member 4) is a member of the GIMAP family of proteins, which are characterized by their GTP-binding AIG1 domain and predominant expression in immune tissues. This protein plays a critical role in regulating lymphocyte survival and homeostasis, particularly in T cells. Functioning as a mitochondrial-associated GTPase, GIMAP4 is implicated in maintaining mitochondrial integrity and modulating apoptosis through interactions with BCL-2 family proteins. Studies in knockout mice reveal its essential role in thymocyte development, where its absence leads to premature T cell death and immune dysfunction.
GIMAP4 has been linked to autoimmune and inflammatory disorders, with altered expression observed in conditions like type 1 diabetes and systemic lupus erythematosus. Its gene resides in a conserved chromosomal region (7q36.1) associated with autoimmune susceptibility. Recombinant human GIMAP4 protein is commonly produced in bacterial or mammalian expression systems for functional studies, enabling researchers to investigate its molecular interactions, structural properties, and regulatory mechanisms. Current research focuses on deciphering its dual roles in immune cell survival and metabolic regulation, offering potential insights into therapeutic strategies for immune-mediated diseases.
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