| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FUNDC2 |
| Uniprot No | Q9BWH2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-189aa |
| 氨基酸序列 | METSAPRAGSQVVATTARHSAAYRADPLRVSSRDKLTEMAASSQGNFEGNFESLDLAEFAKKQPWWRKLFGQESGPSAEKYSVATQLFIGGVTGWCTGFIFQKVGKLAATAVGGGFFLLQLANHTGYIKVDWQRVEKDMKKAKEQLKIRKSNQIPTEVRSKAEEVVSFVKKNVLVTGGFFGGFLLGMAS |
| 分子量 | 47.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人FUNDC2蛋白的虚拟参考文献及其摘要概括，供参考：
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1. **文献名称**：*"FUNDC2 regulates mitochondrial apoptosis by enhancing BAX oligomerization in human colorectal cancer cells"*
**作者**：Chen, L., et al. (2020)
**摘要**：研究通过表达重组人FUNDC2蛋白发现，其在结直肠癌细胞中促进线粒体途径的细胞凋亡，机制涉及增强BAX蛋白寡聚化并激活Caspase-3.提示FUNDC2可能作为癌症治疗靶点。
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2. **文献名称**：*"Recombinant FUNDC2 suppresses lung adenocarcinoma progression via modulating HIF-1α/mTOR signaling"*
**作者**：Zhang, Y., et al. (2018)
**摘要**：利用重组FUNDC2蛋白处理肺腺癌细胞，发现其通过抑制HIF-1α/mTOR通路减少肿瘤细胞增殖和侵袭，并在小鼠模型中验证了FUNDC2的抗肿瘤活性。
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3. **文献名称**：*"Structural insights into the interaction of FUNDC2 with LC3 in selective mitophagy"*
**作者**：Wang, H., et al. (2021)
**摘要**：通过原核系统表达并纯化重组人FUNDC2蛋白，结合X射线晶体学解析其与LC3蛋白的结合结构，揭示了FUNDC2在低氧条件下介导线粒体自噬的关键分子机制。
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4. **文献名称**：*"Optimization of recombinant FUNDC2 production in E. coli for functional studies"*
**作者**：Liu, R., et al. (2019)
**摘要**：报道了一种高效制备重组人FUNDC2蛋白的原核表达体系，通过优化诱导条件和纯化步骤获得高产量可溶蛋白，并验证其体外结合线粒体膜脂质的活性。
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**注意**：以上文献为示例性内容，非真实存在的论文。如需真实文献，建议通过PubMed或Web of Science以“FUNDC2”、“recombinant FUNDC2”为关键词检索。
Recombinant human FUNDC2 (FUN14 domain-containing protein 2) is a mitochondrial protein implicated in cellular energy metabolism and stress responses. Encoded by the FUNDC2 gene, it contains a conserved FUN14 domain, suggesting potential roles in hypoxia adaptation or mitochondrial dynamics. While less studied than its homolog FUNDC1 (known for mitophagy regulation), FUNDC2 is reported to interact with metabolic enzymes like lactate dehydrogenase A (LDHA), influencing aerobic glycolysis—a hallmark of cancer cell proliferation. Its expression correlates with tumor progression, potentially serving as a biomarker or therapeutic target in cancers.
Recombinant FUNDC2 protein, typically produced in bacterial or mammalian expression systems, enables functional studies exploring its molecular mechanisms. Research focuses on elucidating its interactions with mitochondrial membranes, metabolic signaling pathways (e.g., AMPK, HIF-1α), and roles in apoptosis or oxidative stress. Emerging evidence links FUNDC2 dysregulation to neurodegenerative diseases and ischemia-reperfusion injury, expanding its relevance beyond oncology. Its dual localization (mitochondria and cytoplasm) hints at context-dependent functions. Current efforts aim to decode its structural motifs, post-translational modifications, and tissue-specific regulatory networks, offering insights into mitochondrial adaptability in health and disease.
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