| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FTSJ3 |
| Uniprot No | Q8IY81 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-847aa |
| 氨基酸序列 | MGKKGKVGKSRRDKFYHLAKETGYRSRSAFKLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKFMPVSSLIVGVDLVPIKPLPNVVTLQEDITTERCRQALRKELKTWKVDVVLNDGAPNVGASWVHDAYSQAHLTLMALRLACDFLARGGSFITKVFRSRDYQPLLWIFQQLFRRVQATKPQASRHESAEIFVVCQGFLAPDKVDSKFFDPKFAFKEVEVQAKTVTELVTKKKPKAEGYAEGDLTLYHRTSVTDFLRAANPVDFLSKASEIMVDDEELAQHPATTEDIRVCCQDIRVLGRKELRSLLNWRTKLRRYVAKKLKEQAKALDISLSSGEEDEGDEEDSTAGTTKQPSKEEEEEEEEEQLNQTLAEMKAQEVAELKRKKKKLLREQRKQRERVELKMDLPGVSIADEGETGMFSLCTIRGHQLLEEVTQGDMSAADTFLSDLPRDDIYVSDVEDDGDDTSLDSDLDPEELAGVRGHQGLRDQKRMRLTEVQDDKEEEEEENPLLVPLEEKAVLQEEQANLWFSKGSFAGIEDDADEALEISQAQLLFENRRKGRQQQQKQQLPQTPPSCLKTEIMSPLYQDEAPKGTEASSGTEAATGLEGEEKDGISDSDSSTSSEEEESWEPLRGKKRSRGPKSDDDGFEIVPIEDPAKHRILDPEGLALGAVIASSKKAKRDLIDNSFNRYTFNEDEGELPEWFVQEEKQHRIRQLPVGKKEVEHYRKRWREINARPIKKVAEAKARKKRRMLKRLEQTRKKAEAVVNTVDISEREKVAQLRSLYKKAGLGKEKRHVTYVVAKKGVGRKVRRPAGVRGHFKVVDSRMKKDQRAQQRKEQKKKHKRK |
| 分子量 | 123 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FTSJ3蛋白研究的模拟参考文献示例(仅供参考，实际文献需查询学术数据库)：
1. **文献名称**：*Structural and Functional Analysis of Human FTSJ3 as a Ribosomal RNA Methyltransferase*
**作者**：Li, X. et al.
**摘要**：研究解析了重组人FTSJ3蛋白的晶体结构，表明其通过催化核糖体RNA的2'-O-甲基化修饰调控翻译过程，并验证了其酶活性依赖S-腺苷甲硫氨酸(SAM)的辅助因子。
2. **文献名称**：*FTSJ3 Regulates Cell Proliferation via p53 Pathway by Methylating Non-coding RNA*
**作者**：Zhang, Y. et al.
**摘要**：利用重组人FTSJ3蛋白进行功能实验，发现其通过甲基化特定长链非编码RNA(lncRNA)激活p53信号通路，抑制肿瘤细胞增殖，提示其潜在的抑癌作用。
3. **文献名称**：*Recombinant Human FTSJ3 Expression and Interaction with Ribosomal Protein L23*
**作者**：Wang, H. et al.
**摘要**：报道了重组人FTSJ3在大肠杆菌中的高效表达与纯化方法，并利用Pull-down实验证明其与核糖体蛋白L23存在直接互作，可能参与核糖体成熟过程。
4. **文献名称**：*Knockdown of FTSJ3 Impairs Mitochondrial Function via Altered RNA Modification*
**作者**：Chen, L. et al.
**摘要**：通过RNA干扰和重组蛋白回补实验，证明FTSJ3缺失导致线粒体RNA甲基化水平下降，进而引起呼吸链复合物功能异常，揭示其在线粒体代谢中的新角色。
**注意**：以上文献为示例性内容，实际研究请参考PubMed、Web of Science等平台的权威论文。
Recombinant human FTSJ3 (FtsJ RNA 2’-O-methyltransferase 3) is a protein encoded by the FTSJ3 gene, belonging to the conserved methyltransferase superfamily. This enzyme is implicated in RNA modification processes, particularly 2’-O-methylation of ribosomal RNA (rRNA), a post-transcriptional modification critical for ribosome biogenesis and function. Structurally, FTSJ3 contains a conserved S-adenosyl-L-methionine (SAM)-binding domain, which facilitates methyl group transfer during catalysis. Its role in rRNA maturation suggests involvement in regulating translation fidelity and cellular protein synthesis.
Studies highlight FTSJ3's association with cellular proliferation, differentiation, and stress responses. Dysregulation of FTSJ3 has been linked to various pathologies, including cancer and neurodegenerative disorders. For instance, overexpression in certain tumors correlates with enhanced cell survival and chemoresistance, potentially due to altered ribosome activity promoting oncogenic translation. Conversely, reduced FTSJ3 function may impair neuronal RNA metabolism, contributing to neurodevelopmental defects.
Recombinant FTSJ3 protein is typically produced in heterologous systems (e.g., *E. coli* or mammalian cell lines) for *in vitro* studies. It enables mechanistic exploration of methyltransferase activity, substrate specificity, and interaction partners. Emerging research focuses on targeting FTSJ3 for therapeutic interventions, particularly in cancers where ribosome dysregulation drives pathogenesis. Its recombinant form also aids in structural studies to design inhibitors or modulators, advancing both basic and applied biomedical research.
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