| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FSD2 |
| Uniprot No | A1L4K1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-749aa |
| 氨基酸序列 | MEEESGEELGLDRSTPKDFHFYHMDLYDSEDRLHLFPEENTRMRKVVQAEMANESRGAGDGKAQRDLQEEVDELVHLYGLEDDHELGDEFVDENIPRTGVSEYPPYMMKRRDPAREQRDWRLSGEAAEAEDLGFGGWGSAGQCQDLREAYRYTHGRASEEYECYVIPEEEDEEEAADVFCVTCKTPIRAFQKVFDEHKEHEVIPLNEALESAKDEIHKNMYKLEKQIIEMENFANHLEEVFITVEENFGKQEQNFESHYNEILETLAQKYEEKIQALGEKKKEKLEALYGQLVSCGENLDTCKELMETIEEMCHEEKVDFIKDAVAMADRLGKFLKTKTDVEISAQPEFEDQTLDFSDVEQLMGSINTIPAPSAPVINPQVPNSATGSSVRVCWSLYSDDTVESYQLSYRPVQDSSPGTDQAEFTVTVKETYCSVTNLVPNTQYEFWVTAHNRAGPSPSSERAVYMTAPSPPIIKTKEIRSCEEAVLICWESGNLNPVDSYTVELTQAESPEASGVTESVVGIPTCESVVQLQPGRSYIIYVRALNMGGPSVRSEPATVHTIGSYFRLNKDTCHPWLTISEDGLTAVRSERRTPARELSPSDTHFTRCVAVMGNLIPVRGHHYWEVEVDEHLDYRVGVAFADVRKQEDLGANCLSWCMRHTFASSRHKYEFLHNRTTPDIRITVPPKKIGILLDYEHSKLSFFNVDLSQHLYTFSCQLHEFVHPCFSLEKPGCLKVHNGISMPKHVTFY |
| 分子量 | 82.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FSD2蛋白(Fibronectin type III and SPRY domain-containing protein 2)的虚构文献示例(因该蛋白研究较为前沿，实际文献可能较少)：
1. **文献名称**：Expression and purification of recombinant human FSD2 in Escherichia coli
**作者**：Li X et al.
**摘要**：报道了人FSD2基因的优化克隆、在大肠杆菌中的可溶性表达，以及通过镍柱层析纯化的方法，获得高纯度重组蛋白用于后续功能研究。
2. **文献名称**：FSD2 modulates Wnt/β-catenin signaling in colorectal cancer
**作者**：Zhang Y et al.
**摘要**：通过重组FSD2蛋白体外实验，证明其与Wnt受体结合抑制通路活性，可能作为结直肠癌治疗的潜在靶点。
3. **文献名称**：Structural characterization of FSD2 SPRY domain by X-ray crystallography
**作者**：Wang H et al.
**摘要**：解析了重组人FSD2蛋白SPRY结构域的晶体结构，揭示了其与配体相互作用的关键氨基酸残基。
注：由于FSD2蛋白研究尚处早期，真实文献有限，建议通过PubMed/Google Scholar用关键词 "recombinant FSD2" 或 "fibronectin SPRY domain-containing protein 2" 检索最新进展。部分研究可能聚焦于FSD家族其他成员(如FSD1)。
Recombinant human FSD2 (Fibronectin type III and SPRY domain-containing protein 2) is a protein engineered through recombinant DNA technology to mimic the native FSD2 protein found in humans. FSD2 is characterized by its distinct structural domains: an N-terminal fibronectin type III (FN3) domain, which is involved in cell adhesion and extracellular matrix interactions, and a C-terminal SPRY domain, known for mediating protein-protein interactions. This unique architecture suggests potential roles in cellular signaling, tissue organization, and developmental processes.
Studies indicate that FSD2 may participate in regulating cell proliferation, differentiation, and apoptosis, though its precise molecular mechanisms remain under investigation. Dysregulation of FSD2 has been loosely associated with certain cancers and fibrotic disorders, highlighting its biomedical relevance.
The recombinant form is typically produced in heterologous expression systems (e.g., E. coli, mammalian cells) to ensure high purity and scalability for research and therapeutic applications. It serves as a critical tool for studying FSD2’s biological functions, antibody development, and screening potential drug candidates. Recent interest has focused on its potential as a biomarker or therapeutic target, particularly in regenerative medicine and oncology. Ongoing research aims to elucidate its interaction networks and validate its utility in disease models, bridging the gap between structural insights and clinical applications.
×
