| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM43A |
| Uniprot No | Q8N2R8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-423aa |
| 氨基酸序列 | MLPWKKHKFELLAEAPPRQASKPKGYAVSLHYSALSSLARACPEGALSRVGSMFRSKRKKLHITSEDPTYTVLYLGNATTIQARGDGCTDLAVGKIWSKSEAGRQGTKMKLTVSAQGIRMVHAEERALRRPGHLYLLHRVTYCVADARLPKVFAWVYRHELKHKAVMLRCHAVLVSKPEKAQAMALLLYQTSANALAEFKRLKRRDDARHQQQELVGAHTIPLVPLRKLLLHGPCCYKPPVERSRSAPKLGSITEDLLGEQLEQELQEEEEEEQPEGCPEEEENRAAEGDPAEEEAEAQRALVVAMHFECGDLLDTLENGRGEALGGGGGSLGPGAGPPPLLLGSASDMKAELSQLISDLGELSFGNDVRTLQADLRVTRLLSGDSTGSESSIEGGGPDATSATAGDSSRQADGASADEPHSG |
| 分子量 | 72.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM43A蛋白的3篇文献示例(注：以下内容为虚构，仅作格式参考)：
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1. **《重组人FAM43A蛋白的制备及其在肝癌细胞中的抑癌作用》**
*作者：Zhang L, Wang Y, et al. (2021)*
**摘要**：研究通过大肠杆菌系统表达并纯化重组人FAM43A蛋白，发现其过表达显著抑制肝癌细胞HepG2的增殖，并诱导细胞凋亡，可能与抑制PI3K/AKT信号通路相关。
2. **《FAM43A重组蛋白与β-catenin的相互作用及其对Wnt通路调控的机制》**
*作者：Li X, Chen J, et al. (2019)*
**摘要**：利用哺乳动物细胞表达重组FAM43A蛋白，通过免疫共沉淀实验证实其与β-catenin直接结合，并揭示其通过破坏Wnt/β-catenin复合体抑制结直肠癌细胞迁移。
3. **《重组FAM43A蛋白的稳定性优化及其功能验证》**
*作者：Smith K, Johnson R, et al. (2020)*
**摘要**：研究优化了FAM43A蛋白在真核表达系统中的分泌表达条件，证明其通过抑制NF-κB通路降低乳腺癌细胞侵袭能力，为其作为潜在治疗靶点提供依据。
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以上文献示例聚焦于重组蛋白的制备、功能及机制研究。真实文献需通过学术数据库(如PubMed、Web of Science)检索确认。
Recombinant human FAM43A (Family With Sequence Similarity 43 Member A) is a protein of growing interest in biomedical research due to its potential roles in cellular processes and disease associations. Although its precise molecular mechanisms remain unclear, FAM43A is speculated to engage in protein-protein interactions, possibly influencing signaling pathways. Structurally, it contains a conserved domain with predicted leucine-rich repeats (LRR), suggesting involvement in protein recognition or complex formation. The gene encoding FAM43A is ubiquitously expressed, with elevated levels observed in tissues like the brain, liver, and reproductive organs.
Recombinant production of FAM43A typically employs heterologous expression systems such as E. coli or mammalian cell lines (e.g., HEK293), enabling purification via affinity tags for downstream applications. Studies have linked FAM43A to various pathologies, including cancers (e.g., hepatocellular carcinoma, colorectal cancer) and neurological disorders. Notably, it has been identified as an interactor of PI4KB, a kinase involved in phosphoinositide metabolism, hinting at roles in membrane trafficking or lipid signaling. Emerging evidence also connects FAM43A to the PI3K/AKT/mTOR pathway, implicating it in cell survival and proliferation regulation. Its differential expression in disease states highlights potential utility as a diagnostic biomarker or therapeutic target. However, functional characterization remains incomplete, necessitating further research to elucidate its biological significance and mechanistic contributions to health and disease.
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