| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM40B |
| Uniprot No | Q9ULQ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-758aa |
| 氨基酸序列 | MEDPAAPGTGGPPANGNGNGGGKGKQAAPKGREAFRSQRRESEGSVDCPTLEFEYGDADGHAAELSELYSYTENLEFTNNRRCFEEDFKTQVQGKEWLELEEDAQKAYIMGLLDRLEVVSRERRLKVARAVLYLAQGTFGECDSEVDVLHWSRYNCFLLYQMGTFSTFLELLHMEIDNSQACSSALRKPAVSIADSTELRVLLSVMYLMVENIRLERETDPCGWRTARETFRTELSFSMHNEEPFALLLFSMVTKFCSGLAPHFPIKKVLLLLWKVVMFTLGGFEHLQTLKVQKRAELGLPPLAEDSIQVVKSMRAASPPSYTLDLGESQLAPPPSKLRGRRGSRRQLLTKQDSLDIYNERDLFKTEEPATEEEEESAGDGERTLDGELDLLEQDPLVPPPPSQAPLSAERVAFPKGLPWAPKVRQKDIEHFLEMSRNKFIGFTLGQDTDTLVGLPRPIHESVKTLKQHKYISIADVQIKNEEELEKCPMSLGEEVVPETPCEILYQGMLYSLPQYMIALLKILLAAAPTSKAKTDSINILADVLPEEMPITVLQSMKLGIDVNRHKEIIVKSISTLLLLLLKHFKLNHIYQFEYVSQHLVFANCIPLILKFFNQNILSYITAKNSISVLDYPCCTIQDLPELTTESLEAGDNSQFCWRNLFSCINLLRLLNKLTKWKHSRTMMLVVFKSAPILKRALKVKQAMLQLYVLKLLKLQTKYLGRQWRKSNMKTMSAIYQKVRHRMNDDWAYGNGESSQSS |
| 分子量 | 112.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM40B蛋白的3条参考文献示例(请注意，文献为模拟概括，实际研究需通过数据库验证)：
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**1. 文献名称**：*Structural and functional characterization of the FAM40B/STRIPAK complex in cell signaling*
**作者**：Smith A, et al.
**摘要**：研究解析了FAM40B作为STRIPAK复合体关键组分的结构，通过重组表达纯化人源FAM40B蛋白，发现其与PP2A磷酸酶及其他复合体成员相互作用，调控MAPK信号通路的去磷酸化过程。
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**2. 文献名称**：*FAM40B regulates autophagy via interaction with mTORC1 pathway*
**作者**：Chen L, et al.
**摘要**：通过重组FAM40B蛋白的体外实验，证明其与mTORC1复合体的直接结合能力，并揭示FAM40B通过磷酸化修饰调节自噬起始的分子机制，为癌症治疗提供潜在靶点。
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**3. 文献名称**：*Recombinant human FAM40B expression and its role in apoptosis*
**作者**：Wang Y, et al.
**摘要**：报道了FAM40B蛋白在大肠杆菌中的重组表达与纯化方法，功能研究表明过表达FAM40B可通过激活 caspase-3 促进肿瘤细胞凋亡，提示其在细胞死亡中的调控作用。
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**备注**：FAM40B(现称STRIP2)的研究多聚焦于其参与STRIPAK复合体调控细胞骨架重组及信号传导，实际文献需通过PubMed或Web of Science以“FAM40B”或“STRIP2”为关键词检索。
Recombinant human FAM40B protein is a genetically engineered form of the FAM40B (Family with sequence similarity 40 member B) protein, which belongs to a conserved protein family implicated in regulating cellular signaling pathways. FAM40B interacts with protein phosphatase 2A (PP2A) and components of the striatin-interacting phosphatase and kinase (STRIPAK) complex, a multiprotein assembly involved in cell cycle control, cytoskeletal organization, and signal transduction. It plays roles in modulating Hippo, MAPK, and mTOR pathways, influencing cell proliferation, differentiation, and apoptosis.
Structurally, FAM40B contains a putative PP2A-binding domain and conserved motifs for protein-protein interactions. Dysregulation of FAM40B has been linked to cancers, neurodevelopmental disorders, and cardiovascular diseases, highlighting its biological and clinical significance. Recombinant FAM40B is typically produced in prokaryotic (e.g., E. coli) or eukaryotic expression systems to ensure proper folding and post-translational modifications. It is purified via affinity tags (e.g., His-tag) and validated by SDS-PAGE, Western blotting, and functional assays.
This recombinant protein serves as a critical tool for studying FAM40B's molecular mechanisms, identifying binding partners, and screening therapeutic agents targeting its associated pathways. Its applications span basic research, drug discovery, and biotechnological innovations.
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