| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM40A |
| Uniprot No | Q5VSL9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-742aa |
| 氨基酸序列 | MNRKCFEEDFRIHVTDKKWTELDTNQHRTHAMRLLDGLEVTAREKRLKVARAILYVAQGTFGECSSEAEVQSWMRYNIFLLLEVGTFNALVELLNMEIDNSAACSSAVRKPAISLADSTDLRVLLNIMYLIVETVHQECEGDKAEWRTMRQTFRAELGSPLYNNEPFAIMLFGMVTKFCSGHAPHFPMKKVLLLLWKTVLCTLGGFEELQSMKAEKRSILGLPPLPEDSIKVIRNMRAASPPASASDLIEQQQKRGRREHKALIKQDNLDAFNERDPYKADDSREEEEENDDDNSLEGETFPLERDEVMPPPLQHPQTDRLTCPKGLPWAPKVREKDIEMFLESSRSKFIGYTLGSDTNTVVGLPRPIHESIKTLKQHKYTSIAEVQAQMEEEYLRSPLSGGEEEVEQVPAETLYQGLLPSLPQYMIALLKILLAAAPTSKAKTDSINILADVLPEEMPTTVLQSMKLGVDVNRHKEVIVKAISAVLLLLLKHFKLNHVYQFEYMAQHLVFANCIPLILKFFNQNIMSYITAKNSISVLDYPHCVVHELPELTAESLEAGDSNQFCWRNLFSCINLLRILNKLTKWKHSRTMMLVVFKSAPILKRALKVKQAMMQLYVLKLLKVQTKYLGRQWRKSNMKTMSAIYQKVQHRLNDDWAYGNDLDARPWDFQAEECALRANIERFNARRYDRAHSNPDFLPVDNCLQSVLGQRVDLPEDFQMNYDLWLEREVFSKPISWEELLQ |
| 分子量 | 108.02 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM40A蛋白的3篇假设文献的简要列举(注：内容为模拟虚构，实际文献需通过数据库检索)：
1. **文献名称**：*"Functional role of FAM40A in the STRIPAK complex through recombinant protein interaction studies"*
**作者**：Smith J, et al.
**摘要**：本研究通过表达重组人FAM40A蛋白，发现其与STRIPAK复合体中的PP2A磷酸酶和STRN3相互作用，调节细胞骨架重塑和MAPK信号通路活性。
2. **文献名称**：*"Recombinant expression and biochemical characterization of human FAM40A in HEK293 cells"*
**作者**：Zhang L, et al.
**摘要**：报道了在HEK293细胞中高效表达重组人FAM40A蛋白的方法，纯化后经体外实验证实其通过C端结构域介导蛋白互作，并参与调控细胞凋亡。
3. **文献名称**：*"Structural analysis of FAM40A reveals its regulatory role in PP2A-mediated signaling pathways"*
**作者**：Tanaka K, et al.
**摘要**：通过X射线晶体学解析重组FAM40A蛋白的结构，发现其N端螺旋结构对结合PP2A磷酸酶至关重要，并揭示其在癌症细胞迁移中的抑制作用。
(注：实际文献需查询PubMed、Web of Science等数据库，关键词“FAM40A recombinant”或“STRIPAK FAM40A”可能提供相关结果。)
**Background of Recombinant Human FAM40A Protein**
Recombinant human FAM40A (family with sequence similarity 40 member A), also known as STRIP1 (Striatin-interacting protein 1), is a component of the conserved striatin-interacting phosphatase and kinase (STRIPAK) complex. This multi-subunit complex regulates diverse cellular processes, including cell signaling, cytoskeletal organization, and cell cycle progression. FAM40A interacts directly with striatins (STRN, STRN3. STRN4) and acts as a scaffolding protein, facilitating assembly of the STRIPAK complex to modulate downstream signaling pathways, such as the Hippo and MAPK cascades.
Biologically, FAM40A is implicated in maintaining cell polarity, apoptosis, and differentiation. Dysregulation of FAM40A or its associated complex has been linked to cancers (e.g., melanoma, glioblastoma) and neurodevelopmental disorders. Studies suggest its involvement in tumor suppression through mechanisms that control cellular proliferation and metastasis.
The recombinant form of FAM40A is typically produced in *E. coli* or mammalian expression systems, enabling biochemical and functional studies. Its purified form aids in exploring protein-protein interactions, structural analyses, and screening for therapeutic agents targeting STRIPAK-related diseases. Research on FAM40A continues to uncover its precise molecular roles, highlighting its potential as a biomarker or drug target in pathological conditions linked to signaling pathway imbalances.
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