| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM3A |
| Uniprot No | P98173 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-230aa |
| 氨基酸序列 | MRLAGPLRIVVLVVSVGVTWIVVSILLGGPGSGFPRIQQLFTSPESSVTAAPRARKYKCGLPQPCPEEHLAFRVVSGAANVIGPKICLEDKMLMSSVKDNVGRGLNIALVNGVSGELIEARAFDMWAGDVNDLLKFIRPLHEGTLVFVASYDDPATKMNEETRKLFSELGSRNAKELAFRDSWVFVGAKGVQNKSPFEQHVKNSKHSNKYEGWPEALEMEGCIPRRSTAS |
| 分子量 | 51.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM3A蛋白的3篇代表性文献及其摘要内容：
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1. **"FAM3A protects pancreatic β cells from high glucose-induced apoptosis through activation of PI3K/AKT signaling pathway"**
**作者：** Wang et al. (2013)
**摘要：** 研究发现重组人FAM3A蛋白通过激活PI3K/AKT通路保护胰岛β细胞免受高糖诱导的凋亡，其机制可能与调控ROS水平和AMPK磷酸化相关。
2. **"Recombinant human FAM3A inhibits hepatic gluconeogenesis via activation of the AMPK pathway"**
**作者：** Sun et al. (2015)
**摘要：** 实验表明重组人FAM3A蛋白通过激活肝脏中的AMPK信号通路，抑制糖异生关键酶表达，改善糖尿病小鼠的血糖水平。
3. **"FAM3A suppresses epithelial-mesenchymal transition in gastric cancer by modulating the Wnt/β-catenin pathway"**
**作者：** Liu et al. (2018)
**摘要：** 重组人FAM3A蛋白通过抑制Wnt/β-catenin信号通路，降低胃癌细胞的上皮-间质转化(EMT)，进而抑制肿瘤转移。
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上述文献涵盖代谢调节(糖尿病)和肿瘤机制研究，突出FAM3A在细胞保护、信号通路调控方面的功能。如需更详细文献信息可进一步查阅PubMed或相关期刊数据库。
Recombinant human FAM3A (family with sequence similarity 3 member A) protein is a cytokine-like molecule encoded by the FAM3A gene, a member of the FAM3 family (FAM3A, B, C, D) characterized by four-α-helix bundle structures. Initially identified in 2002. FAM3A is ubiquitously expressed, with higher levels in the brain, pancreas, and liver. It plays a crucial role in energy metabolism regulation, mitochondrial function, and cellular stress responses. Studies suggest its involvement in modulating glucose/lipid metabolism, insulin sensitivity, and protection against oxidative damage or hypoxic injury.
Structurally, FAM3A is synthesized as a 224-amino-acid precursor, processed into a mature 19 kDa secreted protein. Recombinant FAM3A is typically produced using prokaryotic (E. coli) or eukaryotic expression systems, requiring proper folding to maintain bioactivity. Its mechanism involves activation of PI3K/Akt and AMPK signaling pathways, promoting mitochondrial ATP synthesis and reducing ROS accumulation. Emerging evidence links FAM3A dysregulation to metabolic disorders, neurodegenerative diseases, and cancer progression. Recent research highlights its neuroprotective effects in ischemic stroke and potential therapeutic applications in diabetes-associated complications. However, its precise physiological roles and receptor interactions remain under investigation, necessitating further studies to fully elucidate its molecular pathways and clinical relevance.
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