| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM38A |
| Uniprot No | Q92508 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-373aa |
| 氨基酸序列 | MKICPFSSKSSIVLALKFRSLIHFEKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLVRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIIPFTAQAYEELSRQFDPQPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYANEKHMLALAPNSTARRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRREQGAGATGFLEWWVIELQECRTDCNLLPMVIFSDKHHGAVRVHRAGHRQVRARILQRDLALHYVRGAAVRGPHPQALPGHLPGAGDSGAGAGGGVVCQAHLPLPLTGDHDQVDS |
| 分子量 | 66.77 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM38A(Piezo1)蛋白的三篇参考文献及其摘要简述：
1. **文献名称**：Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels
**作者**：Coste, B. et al.
**摘要**：该研究首次发现FAM38A(后命名为Piezo1)作为机械敏感性阳离子通道的关键成分。通过重组表达于HEK293细胞，证实其能直接响应机械刺激并激活离子流，揭示其在细胞机械感知中的基础作用。
2. **文献名称**：Structure of the mechanically activated ion channel Piezo1
**作者**：Ge, J. et al.
**摘要**：本研究利用冷冻电镜技术解析了重组人Piezo1蛋白的三维结构，揭示其独特的“叶片状”三聚体构象，阐明了机械力传导与离子通道门控的结构基础，为靶向药物设计提供依据。
3. **文献名称**：Piezo1 links mechanical forces to red blood cell volume
**作者**：Cahalan, S.M. et al.
**摘要**：文章通过重组人Piezo1蛋白功能实验，证明其在调节红细胞体积稳态中的关键作用，发现该通道能感应渗透压变化，突变可导致遗传性溶血性贫血，为相关疾病机制提供新见解。
注：FAM38A为Piezo1的早期命名。上述研究均涉及重组蛋白的表达与功能分析，涵盖基础发现、结构解析及病理机制。
Recombinant human FAM38A protein, also known as Piezo1. is a mechanically activated ion channel critical for converting physical forces into biological signals. It belongs to the Piezo protein family, which includes large transmembrane proteins that form trimeric, non-selective cation channels. FAM38A/Piezo1 is ubiquitously expressed, with notable roles in vascular development, erythrocyte volume regulation, and sensory transduction. Its mechanosensitive properties enable cellular responses to mechanical stimuli like shear stress, stretching, or pressure changes. Structurally, Piezo1 features a unique three-bladed propeller architecture, creating a central ion-conducting pore regulated by membrane tension.
Research on recombinant FAM38A focuses on understanding mechanotransduction mechanisms, particularly in physiological processes such as blood pressure regulation, embryonic development, and tissue homeostasis. Dysregulation of Piezo1 is linked to hereditary xerocytosis, cardiovascular disorders, and certain cancers. Recombinant production of this protein, typically achieved via mammalian expression systems, facilitates functional studies, drug screening, and therapeutic exploration. Its applications extend to disease modeling and the development of targeted therapies for mechano-related pathologies. Ongoing studies aim to unravel its intricate gating mechanism and broader physiological implications.
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