| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM35A |
| Uniprot No | Q86V20 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-835aa |
| 氨基酸序列 | MSGGSQVHIFWGAPIAPLKITVSEDTASLMSVADPWKKIQLLYSQHSLYLKDEKQHKNLENYKVPESIGSPDLSGHFLANCMNRHVHVKDDFVRSVSETQNIESQKIHSSRLSDITSSNMQICGFKSTVPHFTEEEKYQKLLSENKIRDEQPKHQPDICGKNFNTNLFQLGHKCAAVLDLVCSTEKINIGPEVVQRECVPTEYHEIQNQCLGLFSSNAVDKSRSEAAVRKVSDLKISTDTEFLSIITSSQVAFLAQKKDKRRSPVNKGNVNMETEPKASYGEIRIPEENSIQLDGFTEAYESGQNQAYSLELFSPVCPKTENSRIHINSDKGLEEHTGSQELFSSEDELPPNEIRIELCSSGILCSQLNTFHKSAIKRSCTSEDKVGQSEALSRVLQVAKKMKLISNGGDSAVEMDRRNVSEFKSIKKTSLIKNCDSKSQKYNCLVMVLSPCHVKEINIKFGPNSGSKVPLATVTVIDQSETKKKVFLWRTAAFWAFTVFLGDIILLTDVVIHEDQWIGETVLQSTFSSQLLNLGSYSSIQPEEYSSVVSEVVLQDLLAYVSSKHSYLRDLPPRQPQRVNSIDFVELEHLQPDVLVHAVLRVVDFTILTEAVYSYRGQKQKKVMLTVEQAQDQHYALVLWGPGAAWYPQLQRKKGVVLIKAQISELAFPITASQKIALNAHSSLKSIFSSLPNIVYTGCAKCGLELETDENRIYKQCFSCLPFTMKKIYYRPALMTAIDGRHDVCIRVESKLIEKILLNISADCLNRVIVPSSEITYGMVVADLFHSLLAVSAEPCVLKIQSLFVLDENSYPLQQDFSLLDFYPDIVKHGANARL |
| 分子量 | 120.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM35A蛋白的3篇代表性文献及其摘要简述：
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1. **文献名称**: *FAM35A regulates homologous recombination and checkpoint signaling in mammalian cells*
**作者**: Huang et al.
**摘要**: 研究发现FAM35A通过结合RAD51和促进同源重组修复(HRR)维持基因组稳定性。敲除FAM35A导致细胞对DNA损伤敏感，并增强PARP抑制剂疗效，提示其作为癌症治疗靶标的潜力。
2. **文献名称**: *FAM35A interacts with the SHLD3 complex to promote DNA double-strand break repair*
**作者**: Zhang et al.
**摘要**: 揭示了FAM35A与Shieldin复合物(SHLD3)相互作用，在保护DNA断裂末端和调控非同源末端连接(NHEJ)修复途径中起关键作用，其缺失导致端粒脆性和染色体异常。
3. **文献名称**: *FAM35A deficiency compromises cSTING-dependent innate immune signaling*
**作者**: Li et al.
**摘要**: 发现FAM35A通过稳定cGAS-STING通路中的关键蛋白，调控天然免疫响应。其缺失削弱细胞对抗病毒感染的防御能力，并影响肿瘤微环境中的免疫监视。
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以上研究分别从**基因组修复机制**、**免疫调控**等角度解析了FAM35A的功能。如需具体实验方法或疾病模型细节，可进一步扩展查阅。
Recombinant human FAM35A protein, encoded by the *FAM35A* gene (Family With Sequence Similarity 35 Member A, also known as *C6orf80* or *RITA*), is a protein implicated in DNA damage response and genomic stability. Located on chromosome 6q21. FAM35A interacts with key DNA repair factors, including RPA1 and the BRCA1-PALB2 complex, facilitating homologous recombination repair (HRR) of double-strand breaks. Studies suggest its role in maintaining replication fork stability and preventing chromosomal aberrations, linking it to cancer biology, particularly in breast and ovarian cancers where HRR deficiencies are common.
Recombinant FAM35A is typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Researchers employ it to unravel mechanistic insights into DNA repair pathways, characterize its binding partners, and explore its regulatory roles in cell cycle checkpoints. Dysregulation of FAM35A has been associated with increased cancer susceptibility and chemotherapy resistance, making it a potential biomarker or therapeutic target. Its recombinant form also aids in screening small molecules or antibodies aimed at modulating DNA repair processes. Current research focuses on elucidating its post-translational modifications, tissue-specific isoforms, and crosstalk with other DNA repair machinery components, advancing our understanding of genome maintenance mechanisms and precision oncology strategies.
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