| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ELMOD1 |
| Uniprot No | Q8N336 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-334aa |
| 氨基酸序列 | MKHFLRMLIQVCLYFYCKFLWRCLKFVMRKLTGRCELQRICYNTKPGASRTMKIETSLRDSKSKLLQTSVSVHPDAIEKTIEDIMELKKINPDVNPQLGISLQACLLQIVGYRNLIADVEKLRREAYDSDNPQHEEMLLKLWKFLKPNTPLESRISKQWCEIGFQGDDPKTDFRGMGLLGLYNLQYFAERDATAAQQVLSDSLHPKCRDITKEEISKFSKAEWEKKRMDKAIGYSFAIVGINITDLAYNLLVSGALKTHFYNIAPEAPTLSHFQQTFCYLMHEFHKFWIEEDPMDIMEFNRVREKFRKRIIKQLQNPDMALCPHFAASEGLINM |
| 分子量 | 65.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ELMOD1蛋白的假设参考文献示例(仅供参考，实际文献需通过学术数据库检索)：
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1. **《ELMOD1 is a novel Arf6 GTPase-activating protein involved in actin remodeling and cilia function》**
*作者：Smith A, et al.*
**摘要**：本研究首次报道重组人ELMOD1蛋白的体外功能，证明其通过激活Arf6 GTP酶活性调控细胞骨架重塑和纤毛形成。
2. **《Recombinant expression and purification of human ELMOD1: Insights into its GTPase-activating protein specificity》**
*作者：Brown C, et al.*
**摘要**：成功在大肠杆菌系统中表达并纯化重组ELMOD1蛋白，发现其对Arf家族GTP酶的特异性激活作用，为结构研究奠定基础。
3. **《ELMOD1 knockout mice exhibit hearing loss via disrupted cochlear hair cell function》**
*作者：Zhang Y, et al.*
**摘要**：通过重组ELMOD1蛋白的体外功能实验，结合小鼠模型，揭示ELMOD1缺失导致听觉毛细胞ARF信号异常及听力障碍。
4. **《Structural basis of ELMOD1-mediated ARF GTPase hydrolysis》**
*作者：Lee J, et al.*
**摘要**：解析重组人ELMOD1蛋白的晶体结构，阐明其通过保守ELMO结构域催化ARF GTP水解的分子机制。
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**注意**：以上为假设文献，真实文献需通过PubMed、Google Scholar等平台检索关键词(如“recombinant ELMOD1”、“ELMOD1 GTPase”)获取。
Recombinant human ELMOD1 protein is derived from the ELMO domain-containing protein 1 (ELMOD1), a member of the ELMO protein family involved in regulating cellular processes such as cytoskeletal dynamics, intracellular trafficking, and signaling pathways. ELMOD1 specifically acts as a GTPase-activating protein (GAP) for ADP-ribosylation factor (ARF) GTPases, which are critical for membrane remodeling, vesicle transport, and organelle function. Unlike other ELMO family members that primarily interact with DOCK proteins to modulate Rac GTPase signaling, ELMOD1 uniquely targets ARF isoforms, influencing cellular adhesion, migration, and autophagy.
The recombinant form of ELMOD1 is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to ensure high purity and functionality for experimental applications. It serves as a vital tool in biochemical assays, structural studies, and mechanistic investigations of ARF-related pathways. Research on recombinant ELMOD1 has shed light on its role in ciliogenesis, immune responses, and neurological functions, with potential implications in ciliopathies, neurodegenerative disorders, and cancer. Its study also addresses how dysregulation of ARF GTPases contributes to diseases, offering insights into therapeutic targeting. The availability of recombinant ELMOD1 enables detailed analysis of its GAP activity, interactions, and regulatory networks, advancing our understanding of ARF-mediated cellular regulation.
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