| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ELF3 |
| Uniprot No | P78545 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-371aa |
| 氨基酸序列 | MAATCEISNIFSNYFSAMYSSEDSTLASVPPAATFGADDLVLTLSNPQMSLEGTEKASWLGEQPQFWSKTQVLDWISYQVEKNKYDASAIDFSRCDMDGATLCNCALEELRLVFGPLGDQLHAQLRDLTSSSSDELSWIIELLEKDGMAFQEALDPGPFDQGSPFAQELLDDGQQASPYHPGSCGAGAPSPGSSDVSTAGTGASRSSHSSDSGGSDVDLDPTDGKLFPSDGFRDCKKGDPKHGKRKRGRPRKLSKEYWDCLEGKKSKHAPRGTHLWEFIRDILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTYEKLSRAMRYYYKREILERVDGRRLVYKFGKNSSGWKEEEVLQSRN |
| 分子量 | 67.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ELF3蛋白(E74样因子3)的3篇示例参考文献(注：以下文献信息为示例，实际引用需以真实文献为准)：
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1. **文献名称**: *"ELF3 regulates epithelial cell differentiation through transcriptional activation of genes involved in tight junction formation"*
**作者**: Saito Y. et al. (2002)
**摘要**: 该研究揭示ELF3作为转录因子，通过调控紧密连接相关基因(如Claudins和Occludin)的表达，在上皮细胞分化和屏障功能维持中发挥关键作用。实验表明，重组人ELF3蛋白的过表达可增强细胞间连接稳定性。
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2. **文献名称**: *"ELF3 drives oncogenic transformation in colorectal cancer by modulating Wnt/β-catenin signaling"*
**作者**: Holmberg J. et al. (2015)
**摘要**: 研究发现，ELF3在结直肠癌中异常高表达，通过与β-catenin相互作用激活Wnt信号通路，促进肿瘤增殖和侵袭。重组ELF3蛋白的体外实验证实了其对癌细胞迁移能力的影响。
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3. **文献名称**: *"Structural and functional characterization of recombinant human ELF3 protein in inflammatory responses"*
**作者**: Huang L. et al. (2020)
**摘要**: 本文通过重组表达人ELF3蛋白，解析其晶体结构，并证明其在炎症反应中通过调控IL-8等细胞因子的分泌参与免疫调节，为ELF3作为治疗靶点提供依据。
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建议通过PubMed、Google Scholar等平台搜索关键词“recombinant human ELF3 protein”或“ELF3 function”获取真实文献。
Recombinant human ELF3 protein is a biologically engineered form of the E74-like factor 3 (ELF3), a member of the ETS family of transcription factors. ELF3. also known as Epithelial mitogen (Ese-1), plays a critical role in regulating gene expression involved in epithelial cell differentiation, proliferation, and maintenance of tissue-specific functions. It is predominantly expressed in epithelial tissues and interacts with DNA through its conserved ETS domain, modulating target genes associated with cell adhesion, barrier function, and immune responses. Dysregulation of ELF3 has been implicated in various pathologies, including cancers (e.g., gastrointestinal and ovarian tumors) and inflammatory diseases. Its dual role as an oncogene or tumor suppressor, depending on cellular context, makes it a focus of cancer research.
The recombinant ELF3 protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling large-scale purification for functional studies. It serves as a tool to investigate ELF3's molecular mechanisms, such as its interaction with co-regulators (e.g., AP-1. β-catenin) or DNA-binding specificity. Researchers also leverage recombinant ELF3 to explore its potential in regenerative medicine or targeted therapies, including restoring epithelial integrity or inhibiting tumor progression. Structural studies using this protein aid in mapping functional domains and designing small-molecule modulators. Despite progress, challenges remain in fully elucidating its context-dependent roles and therapeutic applications, necessitating further interdisciplinary research.
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