| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ELL2 |
| Uniprot No | O00472 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-640aa |
| 氨基酸序列 | MAAGGTGGLR EEQRYGLSCG RLGQDNITVL HVKLTETAIR ALETYQSHKN LIPFRPSIQF QGLHGLVKIP KNDPLNEVHN FNFYLSNVGK DNPQGSFDCI QQTFSSSGAS QLNCLGFIQD KITVCATNDS YQMTRERMTQ AEEESRNRST KVIKPGGPYV GKRVQIRKAP QAVSDTVPER KRSTPMNPAN TIRKTHSSST ISQRPYRDRV IHLLALKAYK KPELLARLQK DGVNQKDKNS LGAILQQVAN LNSKDLSYTL KDYVFKELQR DWPGYSEIDR RSLESVLSRK LNPSQNAAGT SRSESPVCSS RDAVSSPQKR LLDSEFIDPL MNKKARISHL TNRVPPTLNG HLNPTSEKSA AGLPLPPAAA AIPTPPPLPS TYLPISHPPQ IVNSNSNSPS TPEGRGTQDL PVDSFSQNDS IYEDQQDKYT SRTSLETLPP GSVLLKCPKP MEENHSMSHK KSKKKSKKHK EKDQIKKHDI ETIEEKEEDL KREEEIAKLN NSSPNSSGGV KEDCTASMEP SAIELPDYLI KYIAIVSYEQ RQNYKDDFNA EYDEYRALHA RMETVARRFI KLDAQRKRLS PGSKEYQNVH EEVLQEYQKI KQSSPNYHEE KYRCEYLHNK LAHIKRLIGE FDQQQAESWS |
| 分子量 | 72.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于重组人ELL2蛋白的参考文献摘要整理：
1. **文献名称**: "ELL2 regulates immunoglobulin secretion by B cells through interaction with the PAF1 complex"
**作者**: Park, K. S., & Lu, Q.
**摘要**: 研究发现重组人ELL2蛋白与PAF1复合体相互作用，促进B细胞分化为浆细胞，并调控免疫球蛋白基因的转录延伸和分泌，揭示了ELL2在体液免疫中的关键作用。
2. **文献名称**: "Structural basis for RNA binding and elongation by the ELL2 transcription factor"
**作者**: Miller, T. C., et al.
**摘要**: 通过X射线晶体学分析重组人ELL2蛋白的结构，揭示了其RNA结合域的构象特征，表明ELL2通过招募RNA聚合酶II调控基因表达的延伸过程。
3. **文献名称**: "ELL2 cooperates with WT1 in acute myeloid leukemia by enhancing oncogenic transcriptional programs"
**作者**: Mandal, M., & Mahajan, N.
**摘要**: 研究证明重组人ELL2与癌基因WT1在白血病细胞中形成复合物，激活促癌基因的转录延伸，促进白血病细胞增殖和存活，为靶向ELL2的治疗策略提供依据。
4. **文献名称**: "Expression and purification of recombinant human ELL2 in a mammalian system for functional studies"
**作者**: Johnson, R. B., et al.
**摘要**: 报道了一种在哺乳动物细胞中高效表达和纯化重组人ELL2蛋白的方法，优化后的蛋白具有天然构象和体外转录活性，适用于后续机制研究。
以上文献涉及ELL2的分子功能、结构、疾病关联及重组表达技术，均为假想概括，实际文献需通过学术数据库核实。
Recombinant human ELL2 protein is a genetically engineered version of the Eleven-Nineteen Lysine-Rich Leukemia 2 (ELL2) protein, a member of the ELL family of transcription elongation factors. Naturally expressed in humans, ELL2 plays a critical role in RNA polymerase II-mediated transcriptional elongation by stabilizing the paused polymerase complex and enhancing transcriptional processivity. It is particularly important in B-cell development, plasma cell differentiation, and immunoglobulin secretion. Dysregulation of ELL2 has been linked to cancers, including multiple myeloma and leukemia, underscoring its significance in cellular physiology and disease.
The recombinant form is produced using heterologous expression systems (e.g., *E. coli*, mammalian cells) to ensure high purity and activity for experimental applications. Researchers utilize recombinant ELL2 to study transcriptional regulation mechanisms, protein-protein interactions, and its role in malignancies. Its structural domains, including conserved elongation factor regions, are preserved in the recombinant protein, enabling functional assays such as *in vitro* transcription, chromatin immunoprecipitation, or inhibitor screening. Additionally, recombinant ELL2 serves as a tool for therapeutic target validation and antibody production in translational research. Its availability accelerates studies on transcriptional dysregulation in diseases and supports drug discovery efforts aimed at modulating ELL2-related pathways.
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