| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ELF4 |
| Uniprot No | Q99607 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-663aa |
| 氨基酸序列 | MAITLQPSDL IFEFASNGMD DDIHQLEDPS VFPAVIVEQV PYPDLLHLYS GLELDDVHNG IITDGTLCMT QDQILEGSFL LTDDNEATSH TMSTAEVLLN MESPSDILDE KQIFSTSEML PDSDPAPAVT LPNYLFPASE PDALNRAGDT SDQEGHSLEE KASREESAKK TGKSKKRIRK TKGNRSTSPV TDPSIPIRKK SKDGKGSTIY LWEFLLALLQ DRNTCPKYIK WTQREKGIFK LVDSKAVSKL WGKQKNKPDM NYETMGRALR YYYQRGILAK VEGQRLVYQF KEMPKDLVVI EDEDESSEAT AAPPQASTAS VASASTTRRT SSRVSSRSAP QGKGSSSWEK PKIQHVGLQP SASLELGPSL DEEIPTTSTM LVSPAEGQVK LTKAVSASSV PSNIHLGVAP VGSGSALTLQ TIPLTTVLTN GPPASTTAPT QLVLQSVPAA STFKDTFTLQ ASFPLNASFQ DSQVAAPGAP LILSGLPQLL AGANRPTNPA PPTVTGAGPA GPSSQPPGTV IAAFIRTSGT TAAPRVKEGP LRSSSYVQGM VTGAPMEGLL VPEETLRELL RDQAHLQPLP TQVVSRGSHN PSLLGNQTLS PPSRPTVGLT PVAELELSSG SGSLLMAEPS VTTSGSLLTR SPTPAPFSPF NPTSLIKMEP HDI |
| 分子量 | 70.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ELF4蛋白的3条参考文献示例(基于领域常见研究方向的合理推测，若需实际文献请通过学术数据库进一步检索)：
1. **文献名称**：**"Expression and functional characterization of recombinant human ELF4 in TLR signaling regulation"**
**作者**：Chen, L., Wang, X., & Zhang, H.
**摘要**：本研究成功在大肠杆菌系统中表达并纯化了重组人ELF4蛋白，通过体外实验证实ELF4通过与TLR信号通路中的关键接头蛋白相互作用，调控巨噬细胞的炎症反应，揭示了其在先天免疫中的潜在作用机制。
2. **文献名称**：**"Structural insights into the DNA-binding domain of ELF4 and its role in tumor suppression"**
**作者**：Kimura, S., Patel, R., & Zhao, M.
**摘要**：利用X射线晶体学解析了重组人ELF4蛋白的DNA结合域结构，发现其特异性识别特定ETS基序。功能实验表明，ELF4过表达可抑制癌细胞增殖，提示其作为肿瘤抑制因子的可能，并为靶向治疗提供结构基础。
3. **文献名称**：**"ELF4 reprograms glucose metabolism in T cells via transcriptional regulation of metabolic enzymes"**
**作者**：Gupta, A., Lee, C. F., & Ng, S. B.
**摘要**：通过构建重组ELF4蛋白的体外细胞模型，研究者发现ELF4直接调控糖酵解和线粒体呼吸相关基因的表达，影响T细胞活化后的代谢重编程，为自身免疫疾病及癌症的代谢干预提供了新思路。
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**注**：以上内容为模拟学术文献的示例，实际文献需通过PubMed、Google Scholar等平台以关键词“recombinant human ELF4”或“ELF4 protein function”检索获取。
Recombinant human ELF4 protein is a genetically engineered form of the E74-like factor 4 (ELF4), a transcription factor belonging to the ETS family. ELF4 plays critical roles in regulating gene expression involved in cell cycle progression, immune response, and tumorigenesis. It is particularly noted for its dual functions in both promoting and suppressing cancer, depending on cellular context. Structurally, ELF4 contains a conserved ETS DNA-binding domain that enables sequence-specific interactions with target genes, such as those regulating apoptosis and innate immunity.
The recombinant version is typically produced using bacterial or eukaryotic expression systems, ensuring high purity and biological activity for experimental and therapeutic studies. Research highlights ELF4’s involvement in modulating innate immune signaling pathways, including its interaction with STING (stimulator of interferon genes) to regulate interferon production and antiviral responses. Dysregulation of ELF4 has been linked to cancers, autoimmune disorders, and inflammation, making it a potential biomarker or therapeutic target.
Studies utilizing recombinant ELF4 protein have advanced understanding of its molecular mechanisms, such as post-translational modifications (e.g., phosphorylation) that influence its stability and transcriptional activity. Its recombinant form enables in vitro assays, structural studies, and drug screening, offering insights into diseases driven by ELF4 dysfunction. Ongoing research aims to harness these insights for developing targeted therapies, particularly in oncology and immune-related pathologies.
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