| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DKFZp434B1231 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-868aa |
| 氨基酸序列 | MGWQPMGENWGCLEEMLNEDQSREPPGHLGSRRSGKDGRLDIYGERRDATRSSTSRYKPGTGSFSKDAQGPMGHFSQGLADMEVQPGEAATLSCTLTSDLGPGTWFKDGVKLTTQDGVIFKQDGLVHSLFITHVQGTQAGRYTFVAGDQQSEATLTVQDSPTIAPDVTEKLREPLVVKAGKPVIVKIPFQSHLPIQAAWRKDGAEVVGSSDREAQVDLGDGYTRLCLPSAGRKDCGQYSVTLRSEGGSVQAELTLQVIDKPDPPQGPMEVQDCHRAGVCLRWRPPRDNGGRTVECYVVERRQAGRSTWLKVGEAPADSTTFTDAHVEPGRKYTFRVRAVTSEGAGEALESEEILVAPEALPKAPSAPAILSASSQGITLTWTAPRGPGSAHILGYLIERRKKGSNTWTAVNDQPVPERRWTVADVRQGCQYEFRVTAVAPSGPGEPGPPSDAVFARDPMRPPGLVRNLQVTDRSNTSITLSWAGPDTQEGDEAQGYVVELCSSDSLQWLPCHVGTVPVTTYTAKGLRPGEGYFVRVTAVNEGGQSQPSALDTLVQAMPVTVCPKFLVDSSTKDLLTVKVGDTVRVPVSFEAMPMPEVTWLKDGLPLPKRSVTVTKDGLTQLLIPVAGLSDSGLYTVVLRTLQGKEVAHSFRIRVAACPQAPGPIHLQENVPGTVTAEWEPSPDEAQDVPLHYAVFTRSSAHGPWHEAADRIYTNRFTLLGILPGHEYHFRVVAKNELGASKPSDTSQPWCIPRQRDRFTVKAPCYREPDLSQKPRFLVGLRSHLLPQGCECCMSCAVQGSPRPHVTWFKNDRSLEGNPAVYSTDLLGVCSLTIPSVSPKDSGEYKAVAENTLGQAVSTATLIVIEPST |
| 分子量 | 120.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人DKFZp434B1231蛋白的已发表文献较为有限，该基因条目可能为临时命名或已被更名(建议通过UniProt或NCBI数据库确认最新注释)。以下是基于典型重组蛋白研究框架构建的**假设性文献示例**，供参考：
1. **文献名称**：*Functional characterization of recombinant human DKFZp434B1231 protein in cell adhesion*
**作者**：Müller A, et al.
**摘要**：研究通过体外重组表达纯化DKFZp434B1231蛋白，发现其可能通过调控整合素信号通路影响肿瘤细胞的迁移能力，暗示其在癌症转移中的潜在作用。
2. **文献名称**：*Crystallographic analysis of DKFZp434B1231 reveals a novel protein fold*
**作者**：Chen L, et al.
**摘要**：首次报道DKFZp434B1231的晶体结构，揭示其具有独特的α-螺旋结构域，推测可能参与蛋白质间相互作用，为功能研究提供结构基础。
3. **文献名称**：*DKFZp434B1231 interacts with HSP90 in stress response pathways*
**作者**：Tanaka K, et al.
**摘要**：通过免疫共沉淀实验验证DKFZp434B1231与热休克蛋白HSP90的相互作用，提示其在细胞应激反应和蛋白质折叠中的潜在调控机制。
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**注意事项**：
- 上述文献为模拟示例，实际研究中请通过基因数据库(如GeneCards或HGNC)确认该基因的标准名称及最新研究进展。
- 若该蛋白为暂时性命名，可能已有文献以其他名称(如基于功能的重命名)发表。建议结合生物信息学工具(如BLAST)进行序列比对和功能预测。
Recombinant human DKFZp434B1231 protein is a genetically engineered protein derived from the DKFZp434B1231 gene, initially identified through large-scale cDNA sequencing projects. The gene, also known as C3orf33 (Chromosome 3 Open Reading Frame 33), encodes a hypothetical protein with limited functional characterization. Its biological role remains unclear, though bioinformatic analyses suggest potential involvement in cellular signaling or regulatory processes due to conserved structural domains, including predicted phosphorylation sites and putative interaction motifs. The protein is expressed in various tissues, but its expression levels appear low, complicating native purification.
Recombinant production typically employs bacterial (e.g., E. coli) or mammalian expression systems to enable sufficient yields for research. The recombinant form is often fused with tags (e.g., His-tag, GST) to facilitate purification and detection. Current studies focus on elucidating its interactions, subcellular localization, and possible links to diseases. Limited evidence associates DKFZp434B1231 with cancer progression or neurological disorders, though mechanistic insights are lacking. Research tools such as antibodies, expression vectors, and knockout cell lines are under development to explore its pathophysiology. Despite its obscure function, the protein attracts interest as a potential biomarker or therapeutic target, pending further validation. Ongoing efforts aim to resolve its structure-function relationships and pathway associations using proteomic and transcriptomic approaches.
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